Measurement of Circulating Cell-free DNA (cfDNA) for Diagnosis and Prognosis of Various Diseases and Evaluation of Tissue Damage

Soroka University Medical Center

Status: Completed

Conditions: Correlation of cFDNA During CPB to Laboratories SIRS Parameters and Clinical Outcomes

Treatments: Diagnostic Test: Blood samples

Study type: Observational

Funder types: Other

Identifiers

NCT05094960

SOR4636CTIL

Details and patient eligibility

About

To characterize the changes in circulating cell-free DNA (cfDNA) levels during cardiopulmonary bypass (CPB) and to access their association with outcomes compared to standard scores.

Full description

In a prospective study, the investigators enrolled seventy-one consecutive patients undergoing non-emergent coronary artery bypass grafting. Blood was drawn at baseline, after 20 and 40 minutes on CPB, after cross-clamp removal, and 30 minutes after chest closure. cfDNA was measured in sera by fluorescent method.

The investigators hypothesize that cfDNA measured by will reflect the magnitude of inflammation induced by CPB. Thus, these measurements could serve for the real-time prognosis of patients undergoing cardiac surgery. The investigators correlate cFDNA to laboratories and clinical outcomes.

Enrollment: 71 patients

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Elective coronary artery bypass grafting surgery

Exclusion criteria

• Age < 18
• Non elective surgery
• other than CABG surgery
• Redo CABG