Measurement of Lipogenic Flux by Deuterium Resolved Mass Spectrometry Via Non-invasive Breath Analysis (D2OBREATH)

University Children's Hospital Basel

Status: Completed

Conditions: De Novo Lipogenesis (DNL)

Treatments: Other: Assessment of de novo lipogenesis non-invasively in exhaled breath

Study type: Interventional

Funder types: Other

Identifiers

NCT05274334

2022-00099 ks22Sinues;

Details and patient eligibility

About

This proof of concept- study is to measure de novo lipogenesis (DNL) non-invasively in exhaled breath in overnight-fasted humans by triggering feeding response by deuterium resolved mass spectrometry.

Full description

The study objective is to measure the DNL by ingestion of a single oral dose of 70% 2H2O at 2 g/kg body-water. The subjects will consume a Yoghurt consisting of 300g of 'Excellence Joghurt Nature gezuckert' which contains 410 Kcal, 28g fat, 34g carbohydrates and 9g protein. The exhaled air of the subjects will be measured several times per hour to analyse de novo lipogenesis (DNL) non-invasive by deuterium resolved mass spectrometry.

Enrollment: 5 patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Healthy adult volunteers
• English speaking, or good knowledge of the English language
• Able to understand the study
• Able to give informed consent

Exclusion criteria

• Current pregnancy

Trial design

Primary purpose: Basic Science

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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5 participants in 1 patient group

intervention group

Experimental group

Description:

Assessment of de novo lipogenesis non-invasively in exhaled breath in overnight-fasted humans by triggering feeding response by deuterium resolved mass spectrometry.

Treatment:

Other: Assessment of de novo lipogenesis non-invasively in exhaled breath