Mechanical Stimulation Improve the Fertilization in ICSI Cycle

Tang-Du Hospital

Status: Unknown

Conditions: Fertilization

Treatments: Behavioral: Mechanical Stimulation

Study type: Interventional

Funder types: Other

Identifiers

NCT03810001

201801

Details and patient eligibility

About

Most fertilization failures after ICSI are caused by failure of oocyte activation defects. But, to date, there is no effective method to overcome this obstacle. To investigate the effect of mechanical stimulation on fertilization failures, investigators plan to recruit women undergoing ICSI treatment cycles. The retrieved sibling oocytes from the patients were randomly divided into two groups. The control group conducted the standard ICSI procedure, while the experimental group conducted the modified ICSI procedure.Consequently, the fertilization rate, 2PN (two pronuclei) rate, 1PN rate, oocyte degradation rate,and exploitable embryos on D3 were recorded to evaluate ICSI cycle outcomes.

Full description

Normal fertilization is the critical for the success of ICSI. To investigate the effect of mechanical stimulation on fertilization, investigators plan to recruit women undergoing ICSI treatment cycles. Exclusion criteria: Patients older than 40 years, the maturation oocytes less than 6. The retrieved sibling oocytes from the patient were randomly divided into two groups. The control group conducted the standard ICSI procedure, while the experimental group conducted the modified ICSI procedure. The modified ICSI procedure involved a micromanipulation mechanical stimulation between insertion of the microinjection needle into the oocyte and expulsion of the spermatozoon from the needle to the ooplasm, without additional reagents treatment. Consequently, the fertilization rate, 2PN rate, 1PN rate, oocyte degradation rate, and exploitable embryos on D3 were recorded. The investigators aim to evaluate whether the mechanical stimulation can be used to improve the normal fertilization and embryo quality in ICSI cycles.

Enrollment: 500 estimated patients

Sex: Female

Ages: 22 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• oocytes number ≥ 8;
• women undergoing intracytoplasmic sperm injection cycle.

Exclusion criteria

• maturation oocytes number less than 6.

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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500 participants in 2 patient groups

standard ICSI procedure

No Intervention group

Description:

a single spermatozoon was injected into the ooplasm

modified ICSI procedure

Experimental group

Description:

slight mechanical stimulation before standard ICSI procedure

Treatment:

Behavioral: Mechanical Stimulation