Mechanisms of Anabolic Resistance in Older Humans

Mayo Clinic

Status: Enrolling

Conditions: Aging

Treatments: Other: Exercise

Study type: Observational

Funder types: Other

Identifiers

NCT06617195

24-003329

Details and patient eligibility

About

The purpose of this research is to understand how chronic inflammation affects muscle function and responses to exercise in older adults.

Full description

Inflamed adipose tissue may contribute to blunted exercise response in skeletal muscle of older adults. The objective of this project is to evaluate a hypothesis that inflamed adipose secretes factors that activate inflammatory cascades in skeletal muscle, which may interfere with exercise-responsive molecular pathways and contribute to dysfunctional muscle phenotypes with aging. This project determine how adipose tissue influences skeletal muscle function and anabolic response to exercise in older adults. Young and older adults will complete studies to assess molecular response to acute exercise from protein synthesis rates, mRNA of exercise-responsive genes, and activation of signaling proteins in skeletal muscle. Adipose tissue will be assessed using a combination of non-invasive imaging and biopsy-based molecular phenotyping. The project will determine if acute exercise response is attenuated in older adults with inflamed adipose tissue phenotype.

Enrollment: 120 estimated patients

Sex: All

Ages: 21 to 85 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

Exclusion Criteria:

1. Diabetes or fasting plasma glucose 126 mg/dL
2. Body mass index (BMI) 30 kg/m2
3. Anemia (female subjects hemoglobin of <11 g/dl and male subjects hemoglobin <12 g/dl)
4. Active coronary artery disease or history of unstable macrovascular disease (unstable angina, myocardial infarction, stroke, and revascularization of coronary, peripheral or carotid artery within 3 months of recruitment)
5. Renal failure (serum creatinine > 1.5mg/dl)
6. Chronic active liver disease (AST>144IU/L or ALT>165IU/L)
7. Oral warfarin group medications or history of blood clotting disorders.
8. Smoking
9. Pregnancy or breastfeeding
10. Alcohol consumption greater than 2 glasses/day or other substance abuse
11. Untreated or uncontrolled hypothyroidism
12. Debilitating chronic disease (at the discretion of the investigators)

Trial design

120 participants in 2 patient groups

Young

Description:

Men and women between the ages of 21-35 years will be recruited for this study. All participants will complete an outpatient study day (body composition, blood draw, treadmill test, strength test) and an inpatient study day (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).

Treatment:

Other: Exercise

Older

Description:

Men and women between the ages of 70-85 years will be recruited for this study. All participants will complete an outpatient study day (body composition, blood draw, treadmill test, strength test) and an inpatient study day (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).

Treatment:

Other: Exercise