Mechanisms of Immunosurveillance for Lung Cancer (MechLungCa)

The Washington University

Status: Completed

Conditions: Lung Cancer

Treatments: Other: blood draw

Study type: Observational

Funder types: Other; Other U.S. Federal agency

Identifiers

NCT01710319

201110275

Details and patient eligibility

About

The purpose of this research study is to investigate the differences in "natural killer (NK) blood cells, a type of white blood cell that fights infection in the body, among different types of patients that have lung surgery. The four different groups of patients are:

• smokers with lung cancer
• smokers without lung cancer
• non-smokers with lung cancer
• non-smokers without lung cancer.

Full description

Research has shown that different strains of mice possess varying susceptibility to lung cancer. C3H and C57BL6 mice are highly resistant to lung cancer, whereas A/J and 129 mice are very susceptible to lung cancer. Data from our lab shows that the mice have different numbers of natural killer (NK) cells as well as different characteristics of those cells. C3H and C57BL6 mice have higher numbers of NK cells as well as higher expression of CD11b, whereas A/J and 129 mice have lower numbers and lower expression.

These findings justify parallel investigation of NK cells in human populations resistant and susceptible to lung cancer. Through blood samples, circulating NK cells can be counted and phenotypically analyzed. Smoking can be used as a factor to establish lung cancer risk. Additionally, non-smokers suffering from lung cancer provide an opportunity to investigate whether lung cancer patients have lower abundance of NK cells and lesser expression of CD11b, independent of the effects of smoking.

Objective:

The main goal of this study is to investigate the quantitative and phenotypic differences in circulating NK cells among human populations. Participants will be classified as heavy smokers (HS), non-smokers (NS), those suffering from lung cancer (LC), and those free from lung cancer (NC).

Hypothesis #1: NS/LC participants will have fewer NK cells and lower expression of CD11b compared to HS/NC and NS/NC participants.

Hypothesis #2: NS/LC participants will have more numerous NK cells and higher expression of CD11b compared to HS/LC participants.

Enrollment: 119 patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Age equal or greater to 18 years
• Ability to read and write in English
• Able to participate in the informed consent process

Exclusion criteria

• Known active hepatitis B, hepatitis C, or HIV/AIDs (found in medical record)
• Chemotherapy or radiation therapy within 3 months of enrollment
• Type 1 Diabetes Mellitus
• Rheumatoid arthritis, Lupus, Multiple Sclerosis, or any other autoimmune disease as deemed necessary for exclusion by the Principal Investigator
• Previous organ transplant
• Blood transfusion within 3 months prior to enrollment
• Any previous cancer, excluding a previous lung cancer
• Steroid use within 4 weeks of enrollment

Trial design

119 participants in 4 patient groups

smoker with lung cancer

Description:

patients that smoke greater than 45 pack years or have end organ damage due to cigarette smoking and have lung cancer

Treatment:

Other: blood draw

smoker without lung cancer

Description:

patients that smoke greater than 45 pack years or have end organ damage due to cigarette smoking and do not have lung cancer

Treatment:

Other: blood draw

nonsmoker with lung cancer

Description:

patients that have smoked less than 100 cigarettes in their lifetime and have lung cancer

Treatment:

Other: blood draw

nonsmoker without lung cancer

Description:

patients that have smoked less than 100 cigarettes in their lifetime and do not have lung cancer

Treatment:

Other: blood draw