Metabolic Fate Modifications of Saturated Fats After an Overfeeding (SURNUT)

Human Nutrition Research Centers (CRNH)

Status

Completed

Conditions

Lipid Metabolism

Inflammation

Obesity

Treatments

Dietary Supplement: Hyperlipidic overfeeding

Study type

Interventional

Funder types

Other

Identifiers

NCT00905892

CRNHRA-09-002

Details and patient eligibility

About

The purpose of this study is to determine the partitioning of exogenous lipids in the postprandial period while a study of overfeeding. The method is based on the incorporation of a stable isotopic tracer (d31_palmitic acid, d31_C16) in lipoprotein triglycerides (TG-CHYLOMICRON and TG-VLDL) and in free fatty acids (FFA).

Full description

Healthy overweight and lean young men are subjected to an overfeeding during 56 days which corresponds to a supplement of 761 Kcal/day. During two exploration days (before : D0 et after : D56 overfeeding) they have ingested a breakfast with tracer (d31_palmitic acid, d31_C16, 20mg/kg) and blood and urine samples were collected every hour of each exploration day. The enrichment in deuterium was measured by gas chromatography-organic mass spectrometry (GC-OMS) in palmitic acid pool of lipid fractions.

Enrollment

40 estimated patients

Sex

Male

Ages

18 to 55 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

18 to 55 years old
BMI 18 to 30 kg/m²
Stable physical activity
Safety subject during medical consultation

Exclusion criteria

Medical or surgical history which may affect energy expenditure (renal -cardiovascular -hepatic- endocrine-inflammatory diseases)
Drug use that could affect energy expenditure (steroids, nicotine substitutes, thyroid hormones)
Eating disorder
Intensive sportive activity
Subjects who Smoke
Claustrophobic subjects