Metabolic Profiling of Hematopoietic Stem Cells in Clonal Hematopoiesis (CHIP)

Imelda Hospital, Bonheiden

Status: Enrolling

Conditions: Osteoporosis; Aging; Hip Fractures; Osteoarthritis, Hip; Clonal Hematopoiesis of Indeterminate Potential

Treatments: Other: Blood and bone marrow cell collection

Study type: Observational

Funder types: Other

Identifiers

NCT05246813

20211214

Details and patient eligibility

About

Bone marrow samples will be collected from patients undergoing hip arthroplasty surgery. Blood and bone marrow samples will be used for metabolic profiling and analysis of relevant CHIP mutations. Combined single-cell transcriptomics and mutation-specific single-cell genotyping (biotin-PCR using mutation-targeted primers followed by sequencing) will subsequently be performed. The gene expression profile of wildtype and mutant hematopoietic stem cells will be compared, performing both broad gene set enrichment analysis and targeted analysis of metabolic pathways.

Enrollment: 24 estimated patients

Sex: All

Ages: 55+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Age 65 years and older

Exclusion criteria

• known haematological condition (myelodysplasia, leukemia, cancer)
• inability to provide written informed consent

Trial design

24 participants in 2 patient groups

Osteoporotic hip fracture patients

Treatment:

Other: Blood and bone marrow cell collection

Hip osteoarthritis patients

Treatment:

Other: Blood and bone marrow cell collection