Metabolism of Branched-chain Amino Acids in Monocyte/Macrophage During Sepsis

Sichuan University

Status: Unknown

Conditions: Sepsis Syndrome

Treatments: Other: Metabolic level of BCAAs in monocytes and macrophages

Study type: Observational

Funder types: Other

Identifiers

NCT03842371

2018-081

Details and patient eligibility

About

The current project was designed to examine the metabolic level of branched-chain amino acids in plasm and monocyte/macrophage, and its role in immune dysfunction during sepsis.

Full description

In this study, the investigators will collect the peripheral blood of 1,3,7,14 day after diagnose of sepsis. Then, the concentration of branched-chain amino acids(BCAAs) in monocytes and plasma, and the expression of BCAAs metabolic enzymes in monocytes will be determined. Additionally, the inflammatory cytokines, e.g., Interleukin-1β (IL-1β), Tumor necrosis factor-α (TNF-α), Interleukin-10 (IL-10）, the chemokines macrophage inflammatory proteins 1α and 1β (MIP-1α and 1β) in the peripheral blood and the phagocytic function of monocytes will also be detected. The current project was designed to examine the metabolic level of branched-chain amino acids in plasm and monocyte/macrophage, and its role in immune dysfunction during sepsis.

Enrollment: 90 estimated patients

Sex: All

Ages: 18 to 70 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• patients diagnosed with sepsis, with or without type 2 diabetes
• healthy volunteers

Exclusion criteria

• patients with immunodeficient diseases
• patients who accept glucocorticoid or immunosuppressant
• patients with an age younger than 18 years
• patients who cannot provide the informed consent

Trial design

90 participants in 3 patient groups

Sepsis with type 2 diabetes

Description:

Sepsis patients with type 2 diabetes

Treatment:

Other: Metabolic level of BCAAs in monocytes and macrophages

Sepsis without type 2 diabetes

Description:

Sepsis patients without type 2 diabetes

Treatment:

Other: Metabolic level of BCAAs in monocytes and macrophages

Volunteers

Description:

Healthy volunteers

Treatment:

Other: Metabolic level of BCAAs in monocytes and macrophages