Metabolome and Microbiomic in Fecal Samples (MicroMet)
Status
Conditions
Details and patient eligibility
About
The purpose of this study is to evaluate the effects of storage and handling on fecal samples collected for metabolomic, proteomic and microbiomic and analysis.
Full description
Background
Human fecal collection for clinical or research studies usually occurs at the residence of the subject, an environment not equipped with measures to accommodate proper preparation and storage of samples for microbiome and metabolome analysis.
Aims
To evaluate the effects of storage and handling on fecal samples collected for metabolomic, proteomic and microbiomic and analysis.
Methods
Fecal samples will be collected within several minutes of voiding from healthy infants at the time of diaper change at day-care center.
Samples will be divided into 7 tubes, and subjected to different storage temperatures, before preforming microbial analysis by DNA sequencing, and metabolome analysis by H1NMR spectroscoty.
Enrollment
Sex
Ages
Volunteers
Inclusion and exclusion criteria
Inclusion Criteria:
- healthy
- age 12-20 months
Trial design
4 participants in 1 patient group
Trial contacts and locations
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Data sourced from clinicaltrials.gov
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