Metabolomics Approach After Acute Intake of Grumixama Juice

University of Sao Paulo

Status: Completed

Conditions: Healthy

Treatments: Dietary Supplement: Intake of grumixama juice

Study type: Interventional

Funder types: Other

Identifiers

NCT02790658

17449613.8.0000.0067

Details and patient eligibility

About

Investigate the bioavailability of the main flavonoid, their colonic transformation and untargeted metabolites by an metabolomic approach following acute intake of a grumixama juice by humans.

Full description

The grumixama is a sweet and little cherry native of the South and Southeast regions of the Atlantic Forest of Brazil rich in phenolic compounds, mainly anthocyanins and ellagitannins. The objective of this study is Investigate the bioavailability of the main flavonoid, their colonic transformation and untargeted metabolites by an metabolomic approach following acute intake of a grumixama juice by humans.

Fifteen healthy subjects consumed grumixama juice at single dose, and urine and plasma samples were collected at different time points over 24 h period. The metabolites were analyzed by LC-ESI-MSn, LC-Q-TOF plasma and urine were also analyzed using untargeted metabolomic approach for amino acids and organic acids by GCMS.

Enrollment: 15 patients

Sex: Female

Ages: 18 to 49 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Not have medical historical of cardiovascular, gastrointestinal, hepatic, renal, thyroid and/or diabetes dysfunction
• Not be alcohol addicts, not use vitamins and other supplements
• Not are using any kind of medication which affect the digestion and absorption of food, not be smokers
• Not are pregnant.

Exclusion criteria

• Have medical historical of cardiovascular, gastrointestinal, hepatic, renal, thyroid and/or diabetes dysfunction
• Be alcohol addicts, use vitamins and other supplements
• Are using any kind of medication which affect the digestion and absorption of food, be smokers
• Are pregnant.

Trial design

15 participants in 1 patient group

Grumixama Juice

Other group

Description:

Juice of grumixama purple fruit (Eugenia brasiliensis Lam.), which is good source of anthocyanins and ellagitannins. It was made with filtered water and sanitized fruits, with a blender. It was administered in single dose of 0.97 mg of anthocyanins and of 4.71 mg of ellagitannins per mL of juice. Which volunteers ingested 10 mL of juice per each Kg body weight. The metabolomic approach of plasma and urine samples following acute intake of grumixama juice was done by the collection of blood samples and urine, following the intake of grumixama juice.

Treatment:

Dietary Supplement: Intake of grumixama juice