Methodological Evaluation of Gingival Crevicular Fluid Volume and Elastase Level

Baskent University

Status: Completed

Conditions: Chronic Periodontitis

Treatments: Other: Gingival crevicular fluid collection with filter paper

Study type: Interventional

Funder types: Other

Identifiers

NCT02676362

08/05

Details and patient eligibility

About

The mechanisms underlying the formation and composition of gingival crevicular fluid (GCF) and its flow into and from periodontal pockets are not understood very well. The aim of this study was to evaluate the length of sampling time and sequential sampling of GCF neutrophil elastase (NE) enzyme activity by using intracrevicular and orifice methods.

Full description

Twenty adults (mean age of 41.8 years. ranged 31-60 years. 18 males. 2 females) with chronic periodontitis were enrolled and all completed the 3-day study. Three-hundred-sixty samples of GCF were harvested from the interproximal sites of each of the two maxillary non-molar teeth according to the orifice method. Each site was sampled three times with 1-min intervals between repeat samples. By using intracrevicular method, the other 360 samples of GCF were collected on the same sites following 10-minutes interval. In first day, 2nd day and 3rd day the length of sampling time in seconds and order were 5. 10. 30; 10. 30. 5 and 30. 5. 10. respectively. GCF elastase activity was determined by hydrolysis of neutrophil specific substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide and was expressed as concentration (microU/microl) and total enzyme activity (microU).

Enrollment: 20 patients

Sex: All

Ages: 31 to 60 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• no history of systemic disease;
• no history of antibiotics and/or anti-inflammatory drugs within the past 6 months;
• no received any oral hygiene prophylaxis and scaling and root planing within the past 6 months;
• at least 2 interproximal sites exhibiting higher than 5 mm probing depth, radiographic bone loss and gingival index of 2 (with preference of two maxillary non-molar teeth)

Exclusion criteria

• with metabolic diseases
• pregnancy, lactation etc.

Trial design

Primary purpose: Diagnostic

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Double Blind

---

20 participants in 3 patient groups

the first day

Experimental group

Description:

Gingival crevicular fluid collection with filter paper the length of sampling time in seconds: 5., 10., 30.

Treatment:

Other: Gingival crevicular fluid collection with filter paper

the 2nd day

Experimental group

Description:

Gingival crevicular fluid collection with filter paper the length of sampling time in seconds: 10., 30., 5.

Treatment:

Other: Gingival crevicular fluid collection with filter paper

the 3rd day

Experimental group

Description:

Gingival crevicular fluid collection with filter paper the length of sampling time in second: 30., 5., 10.

Treatment:

Other: Gingival crevicular fluid collection with filter paper