Methylene Blue Intra-vital Nerve Staining

National Taiwan University

Status: Unknown

Conditions: Neurologic Findings

Treatments: Diagnostic Test: Methylene blue intradermal injection

Study type: Interventional

Funder types: Other

Identifiers

NCT05547685

201904083MINA

Details and patient eligibility

About

In previous studies, we know methylene blue (MB) stains nerves, and oxidized MB has near-infrared (NIR) fluorescence for fluorescent observation. In this trial we using MB on human skin and combining MB and third-harmonic generation imaging (THG) to label cutaneous nerve fibers.

Full description

Intravital images were obtained before injection and then taken at 0.5, 1, 1.5, 2, 2.5, and 3 h after intradermal 0.03% (0.3 mg/mL, 1500μM) MB (10mg/mL "Astar") injection using a 0.5-mL 29-gauge insulin syringe needle (Becton, Dickinson and Company, USA). The participant lay down and the skin was imaged 10 cm above the ankle in the lower leg and above the wrist in the middle forearm; three sites in each limb were randomly selected for each time point. SHG or THG captured MB signals with two-photon excitation microscope at the Molecular Biomedical Imaging Research Center of National Taiwan University.

Enrollment: 5 estimated patients

Sex: All

Ages: 20 to 70 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Healthy Volunteers

Exclusion criteria

• used serotonin agonist medication

Trial design

Primary purpose: Diagnostic

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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5 participants in 1 patient group

Methylene blue intradermal injection

Experimental group

Description:

Methylene blue intradermal injection

Treatment:

Diagnostic Test: Methylene blue intradermal injection