Micro Ribonucleic Acid (miRNA) Expression and Inflammatory Factors in Patients With Parkinson's and Periodontal Disease

Medipol University

Status: Not yet enrolling

Conditions: Periodontal Diseases Inflammation

Treatments: Other: Saliva and Serum

Study type: Interventional

Funder types: Other

Identifiers

NCT07099378

106

Details and patient eligibility

About

MicroRNAs are important regulators of the immune and inflammatory response to bacterial pathogens during periodontal disease.The aim of this study was to evaluate the possible miRNA type for Parkinson's in saliva of orally healthy individuals.

Full description

A total of 51 individuals, 35 systemically healthy and 16 with Parkinson's disease and both dental and periodontal health, were included in the study.Clinical periodontal parameters, DMFT data and salivary flow rates were recorded.Unstimulated saliva samples were taken from each participant and miRNA was evaluated by RT-qPCR method.ELISA method was used to measure TNF α values in collected saliva and serum samples.

Enrollment: 51 estimated patients

Sex: All

Ages: 18 to 65 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• dentally and periodontally healthy

  • Systemically healthy with no uncontrolled chronic and/or autoimmune disease
  • No systemic, neurological, or autoimmune disease in addition to Parkinson's disease.

Exclusion criteria

• history of regular use of systemic antibiotics anti-inflammatory, or antioxidant drugs (previous 3 months)
• nonsurgical periodontal treatment (previous 6 months)
• surgical periodontal treatment (previous 12 months)
• presence of<20 teeth
• current medications affecting gingival health (calcium channel blockers, phenytoin, cyclosporine, and hormone replacement therapy)
• diabetes
• diagnosis of rheumatoid arthritis
• pregnancy
• lactating
• smoking
• excessive alcohol consumption.

Trial design

Primary purpose: Prevention

Allocation: Randomized

Interventional model: Single Group Assignment

Masking: None (Open label)

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51 participants in 2 patient groups

Active Comparator

Active Comparator group

Description:

Collection of patients' saliva and serum and analysis of sample molecules.

Treatment:

Other: Saliva and Serum

Other: Saliva and Serum

Experimental

Experimental group

Description:

ELISA method will be used to measure TNF-α values in collected saliva and serum samples.RT-qPCR based miRNA gene expression analyses will be performed with 2-step qRT-PCR Kits (Thermofisher Scientific, MA, USA).

Treatment:

Other: Saliva and Serum

Other: Saliva and Serum