Microarchitecture of Vitreoretinal Contact

The S.N. Fyodorov Eye Microsurgery State Institution

Status: Completed

Conditions: Vitreo-Retinal Adhesion; Vitreoretinal Detachment

Treatments: Other: scanning electron microscopy

Study type: Interventional

Funder types: Other

Identifiers

NCT04802889

124576

Details and patient eligibility

About

Investigation of the vitreoretinal contact microarchitecture in the macular and paramacular zones of the human retina.

Full description

Purpose: to study the features of the macular ILM, to study the features of the preretinal structures of the vitreous body of the Central retinal region, morphofunctional features of preretinal hyalocytes, and the mechanism of vitreoretinal adhesion.

Study Protocol: examination of donor human eyes obtained from an eye Bank after sampling material for corneal transplantation.

Research method: scanning electron microscopy (SEM) of the central retina (macular and paramacular zone): ILM, preretinal vitreous.

Scientific hypothesis: vitreoretinal adhesion is provided not by laminin and fibronectin, but by the interweaving of vitreous collagen fibers between the epiretinal vitreous (more firmly fixed to the retinal surface) and the cortical vitreous.

Expected results: determination of ILM thickness in the macular zone, microanatomy of preretinal vitreous strutcures, and possible mechanism of vitreoretinal adhesion, establishing the possibility of intravital synthesis of collagen fibers synthesis in the adult vitreous.

Enrollment: 20 patients

Sex: All

Ages: 35 to 45 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• eyes from donors without concomitant multiple organ pathology and ophthalmic pathology

Exclusion criteria

• eye injury, age over 45 years, concomitant multiple organ pathology, previous eye diseases

Trial design

Primary purpose: Basic Science

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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20 participants in 1 patient group

cadaver eyes

Experimental group

Description:

The Central region of the retina and preretinal structures of cadaver eyes are examined

Treatment:

Other: scanning electron microscopy