Microbial Epidemiology and Chlorhexidine Suscebtibily of Oropharyngeal and Intestinal Colonization (OroColi-HS)

Assistance Publique - Hôpitaux de Paris

Status: Completed

Conditions: Oropharyngeal Gram-negative Bacilli Colonization

Treatments: Other: oropharyngeal swabbing; Other: rectal swabbing

Study type: Observational

Funder types: Other

Identifiers

NCT02840656

P140907

Details and patient eligibility

About

In this prospective observational study we aim to determine the prevalence of oropharyngeal and rectal Gram-negative bacilli colonization in healthy subjects, and their susceptibility to chlorehexidine. We plan to recruit 100 healthy volunteers secondary endpoints are to determine the phylogentic characteristics of E. coli isolates; to compare the phylogentic characteristics of oropharyngeal and rectal of predominant GNB colonization.

Full description

In this prospective observational study we aim to determine the prevalence of oropharyngeal and rectal Gram-negative bacilli colonization in 100 healthy subjects, and their susceptibility to chlorehexidine. Each participant will undego a oropharyngeal swab collection and a rectal swab at day 0. The Gram-negative bacilli colonization will be identified, E. coli isolates will be studied in terms of phylogeny; gram-negative isolates will be studied in terms of antimicrobial and chlorhexidine susceptibility. The primary objective is to determine the prevalence of oropharyngeal Gram-negative bacilli colonization. The secondary objectives are to determine the chlorhexidine and antimicrobial susceptibility of such colonization; to determine the phylogentic characteristics of oropharyngeal and rectal predominant E. coli isolates; to compare the phylogentic characteristics of oropharyngeal and rectal E. coli isolates.

Enrollment: 100 patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• age > 18 yo
• personal history of neck or face irradiation
• no history of gastrointestinal disease
• no symptoms of immunosuppression
• no antibiotic therapy in the previous month
• no hospitalization in the 3 months preceding inclusion
• medical exmination prior to inclusion

Exclusion criteria

• affiliated to the social security
• The refusal of a patient to participate in a study

Trial design

100 participants in 1 patient group

healthy adult subject

Description:

oropharyngeal and rectal swabbing to collect Gram-negative bacilli

Treatment:

Other: oropharyngeal swabbing

Other: rectal swabbing