Microbiological Evaluation of Single Versus Multiple Visits Regeneration Using MALDI-TOF Mass Spectrometry

Nourhan M.Aly

Status and phase

Terminated

Phase 2

Conditions

Pulp Necroses

Tooth, Nonvital

Treatments

Biological: Single-visit regeneration protocol

Biological: Multiple-visits regeneration protocol

Study type

Interventional

Funder types

Other

Identifiers

NCT04533074

MALDI-TOF MS regeneration

Details and patient eligibility

About

Aim of the study is to assess canal disinfection using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) in single visit and multiple visit regeneration protocols, and to assess clinical and radiographic outcomes of single visit and multiple visit regeneration protocols.

Full description

Thirty patients with necrotic immature permanent teeth showing periapical lesions will be randomly divided in two groups: group (1) single visit regeneration and group (2) multiple visits regeneration protocol, in which calcium hydroxide will be applied for one week or more according to resolution of signs and symptoms. Dentin debris collected on a file will be taken as microbiologic sample to be cultured and examined by MALDI-TOF MS. This will be performed after the access cavity preparation and after irrigation in both groups, these samples will be labelled A and B, respectively. In group (2) there will be a third sample labeled C which will be taken after the removal of the intra canal calcium hydroxide.

Enrollment

16 patients

Sex

All

Ages

8 to 18 years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Immature permanent upper anterior teeth.
Necrotic teeth confirmed by sensibility test.
Teeth with periapical lesions confirmed by periapical radiograph using paralleling device.

Exclusion criteria