Microbiota Upper Respiratory Tract (ProCure)

Antwerp University Hospital (UZA)

Status: Completed

Conditions: Microbiota Upper Respiratory Tract

Treatments: Other: no intervention, compare URT microbiome healthy control group-chronic rhinosinusitis patients

Study type: Observational

Funder types: Other

Identifiers

NCT02933983

B300201524257

Details and patient eligibility

About

The aim of this study is to investigate whether the microbiota in the upper respiratory tract (URT) (nose, nasopharynx and sinuses) of chronic rhinosinusitis (CRS) patients is disturbed compared to healthy individuals. Therefore, bacterial DNA from swabs, aspirates and tissue will be isolated via commercially available DNA extraction kits, followed by Illumina MiSeq sequencing in order to identify the bacterial species present in these samples. Special attention will go to microbial species that are overrepresented in the healthy populations as potential health promoting microbes (i.e. probiotics). A better understanding of the URT microbiome might help us to better understand the pathology of CRS and might help to develop new microbiota-based strategies for CRS.

Enrollment: 325 patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

Exclusion Criteria healthy population:

• no use of antibiotics in the past year
• no acute or chronic airway infections
• no cancer
• no autoimmune diseases
• pregnant women

Exclusion Criteria CRS population:

--> no exclusion criteria

Trial design

325 participants in 2 patient groups

Control group (healthy)

Description:

Participants who do not suffer from acute or chronic airway infections

Treatment:

Other: no intervention, compare URT microbiome healthy control group-chronic rhinosinusitis patients

CRS patients

Description:

Patients who suffer from chronic rhinosinusitis

Treatment:

Other: no intervention, compare URT microbiome healthy control group-chronic rhinosinusitis patients