Microgravity Effects on Co-cultured Vascular Cells Types

University of Florida

Status: Completed

Conditions: Microgravity Exposed Endothelial Cells

Treatments: Biological: Blood volume collected specifically for this study

Study type: Observational

Funder types: Other; Other U.S. Federal agency

Identifiers

NCT03144492

16-2016GL_FP-0011 (Other Grant/Funding Number)

IRB201700297

Details and patient eligibility

About

The goal of this study is to assess the affect of ground based microgravity on co-cultured vascular cell types. Our goal is to better understand the cross-talk between vascular cell types grown under conditions such as microgravity as is seen in space. In this pilot study, the investigator proposes to assess changes in cell phenotype and function in microgravity as compared to normal gravity.

Full description

This study builds upon previously reported space flight data in GenLab, as well as our own published data whereby the investigator evaluate the effect of microgravity on the differentiation and functional potential of circulating stem cells. The goal of this work is to leverage the powerful genetic analyses done in the SPHINX study and look at the consequences these changes have on cell-cell communications essential for vascular homeostasis, for a possible correlation to cardiovascular deconditioning. The application of this work is targeted at possible "cell based" countermeasures to address the negative effects of microgravity on the vasculature.

Enrollment: 2 patients

Sex: All

Ages: 21 to 50 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Self-reported healthy individuals
• Willingness to have 50mls of whole blood collected

Exclusion criteria

• Unwillingness to have blood used in stem cell research

Trial design

2 participants in 1 patient group

self-reported healthy individuals

Description:

Self-reported healthy individuals avoiding blood draw on the days participants are feeling "under the weather"or sick. In that case, study team can delay the blood draw until the subject feels better, but this would not preclude anyone from participating.

Treatment:

Biological: Blood volume collected specifically for this study