microRNAs Role in Pre-eclampsia Diagnosis

Cairo University (CU)

Status: Completed

Conditions: Preeclampsia

Study type: Observational

Funder types: Other

Identifiers

NCT03562715

BC (1870)

Details and patient eligibility

About

Pre-eclampsia is one of the most threatening pregnancy complications. So far neither a secure, competent therapy for PE nor effective biomarkers for a premature discovery has been achieved.The aim of our study was to identify miRNAs 136, 494 and 495 genes expression in exosomes of peripheral blood compared to umbilical cord mesenchymal stem cells conditioned media released exososomes in patients with PE, as valuable markers for PE early prediction.

Enrollment: 200 patients

Sex: Female

Ages: 23 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

To have an uncomplicated pregnancy:

1. gestational age at venipuncture between 20 - 42 weeks
2. no medical, obstetrical, or surgical complications
3. absence of labor at the time of venipuncture
4. delivery of a normal term (≥ 37 weeks) neonate whose birth weight was between the 10th and 90th percentile for gestational age

Exclusion criteria

Patients with chronic hypertension, renal disease, and fetuses affected with congenital anomalies were excluded

Trial design

200 participants in 2 patient groups

Control group

Description:

Blood samples were collected from 100 control with normal pregnancies. Thirty fresh umbilical cord samples of women with healthy pregnancies (n=15) were retrieved during caesarean deliveries and umibilical cord mesenchymal stem cells (UCMSCs) were isolated from Wharton jelly.

Preeclampsia group

Description:

Blood samples were collected from 100 patients with PE. Thirty fresh umbilical cord samples of PE patients (n=15) were retrieved during caesarean deliveries and UCMSCs were isolated from Wharton jelly.