miRNA Machinery in Melanoma, Melanoma Metastases and Benign Melanocytic Naevi

Ruhr University of Bochum

Status: Completed

Conditions: Nevi; Cutaneous Melanoma

Study type: Observational

Funder types: Other

Identifiers

NCT01444560

microRNA4

Details and patient eligibility

About

MicroRNAs (miRNAs) are very small endogenous RNA molecules about 22-25 nucleotides in length, capable of post-transcriptional gene regulation. miRNAs bind to their target messenger RNAs (mRNAs), leading to cleavage or suppression of target mRNA translation based on the degree of complementarity. miRNAs have recently been shown to play pivotal roles in diverse developmental and cellular processes and linked to a variety of skin diseases and cancers. In the present study, the investigators examines the expression profiles of miRNA machinery components such as miRNA maturation and transport factors, microprocessor complex and RISC subunits in cutaneous melanoma, cutaneous melanoma metastases and benign melanocytic nevi.

Full description

Patients with cutaneous melanoma, cutaneous melanoma metastases and benign melanocytic nevi were included in the study. Punch biopsies were harvested from the center of the tumors. The miRNA machinery components were detected by quantitative real-time reverse transcriptase polymerase chain reaction.

Enrollment: 21 patients

Sex: All

Ages: 1+ year old

Volunteers: No Healthy Volunteers

Inclusion criteria

• cutaneous melanoma
• cutaneous melanoma metastases
• benign melanocytic nevi

Exclusion criteria

• other forms of malignancy

Trial design

21 participants in 3 patient groups

Cutaneous Melanoma

Cutaneous Melanoma Metastases

Benign Melanocytic Nevi