Mitochondrial Energy Production in ART (Assisted Reproductive Technology ) (Mitenergy)

IVI Madrid

Status

Completed

Conditions

Female Infertility Due to Advanced Maternal Age

Study type

Observational

Funder types

Other

Identifiers

NCT02727400

1512-MAD-064-JGPROTOCOLOf-151

Details and patient eligibility

About

Oocyte maturation requires a lot of energy, which is provided by the mitochondria via the synthesis of ATP. The majority of patients with advanced maternal age (AMA) have poor egg quality. One of the reasons depends on oxidative phosphorylation of Pyruvate to undergo maturation; on the contrary, the cells of the cumulus (CC) show great activity glycolytic so that these cells are able to provide ATP(adenosine triphosphate and energy substrates to the oocyte. The goal of this work is to analyze the mitochondrial function and energy production in oocyte donors compared with a group of women of advanced maternal age subject to a same ovarian stimulation Protocol

Enrollment

60 patients

Sex

Female

Ages

18 to 46 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Controls: Women between 18-35 years old who are oocyte donors and fulfill the requirements of the our program of oocyte donation according to the Spanish Law of Human Reproduction
Cases: infertile women undergoing IVF due to advanced maternal age (>38 years)

Exclusion criteria

For controls: Women with antral follicle count >20 or <6 follicle per ovary or with pathologies which, in the opinion of the investigator, may interfere with the treatment of ovarian stimulation

Severe hypersensitivity to drugs of similar structure
In the case of associated pathologies which can be considered as exclusion criteria on the part of the researcher are endocrine disruption and multi-follicular ovaries with risk of OHSS (Ovarian hyperstimulation syndrome)
Be positive in serology for syphilis, toxoplasmosis, rubella, gonorrhea, hepatitis B, hepatitis C and HIV and lack of personal history and family of hereditary diseases.

Cases