Mitochondrial Oxygraphy on Subcutaneous and Peri-visceral Adipose Tissue: Influence of Body Composition (OxMiTiAd)

University Hospital, Clermont-Ferrand

Status: Terminated

Conditions: Bariatric Surgery Candidate; Digestive Organs--Cancer; Parietal Region Trauma

Treatments: Procedure: adipose surgery

Study type: Interventional

Funder types: Other

Identifiers

NCT05417581

RBHP 2020 RICHARD

2020-A03309-30 (Other Identifier)

Details and patient eligibility

About

The objective of this study is to adapt the oxygraphy technique on human adipocytes and to characterize respiration measurements according to patients' body mass index and white adipose tissue localization (subcutaneous vs visceral).

Full description

During digestive surgery: oncological and bariatric surgery, it is possible to collect a small volume of subcutaneous and epiplonic adipose tissue (10-20 cc). We wish to collect these samples in order to develop the technique of oxygenography on permeabilized adipocytes. In order to characterize these measurements, they will be related to the clinical data of the patients usually collected in the framework of the preoperative assessment including the body mass index (BMI). The measured adipocyte data will thus be normalized to three BMI classes: ≤ 20, > to 20 < 30, and ≥ to 30 kg∙m-2.

Primary end point is to validate the measurement of mitochondrial respiration under oxidophosphorylating conditions by determining indicators of the distribution of mitochondrial respiration values (mean, standard deviation, skewness, kurtosis, coefficient of variation) as a function of patients' body mass index.

Enrollment: 67 patients

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patients undergoing oncological, bariatric, parietal surgery
• Patients able to give an informed consent
• Patients affiliated to an Health Care insurance
• Patients aged over 18

Exclusion criteria

• Patients under guardianship, curatorship or deprived of freedom
• Pregnant or breastfeeding women
• Participation refusal
• Patients under chemotherapy
• Patients with peritonitis and infectious parietal processes

Trial design

Primary purpose: Health Services Research

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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67 participants in 1 patient group

All patients

Experimental group

Description:

a sample of adipose tissue will be collected during the scheduled surgery

Treatment:

Procedure: adipose surgery