MMP-9 Expression in Permanent Mature Teeth With Symptomatic Pulpitis

Eglė Gvazdaitytė

Status: Completed

Conditions: Pulpitis

Treatments: Procedure: Coronal pulpotomy

Study type: Interventional

Funder types: Other

Identifiers

NCT03807674

BEC-LSMU(R)-01

Details and patient eligibility

About

Matrix metallopeptidase-9 (MMP-9) expression was compared in healthy and inflamed pulp and the outcome of coronal pulpotomy in teeth with symptomatic pulpitis was assessed. After procedure blood samples were examined using Elisa kit.

Full description

Introduction: Widely used diagnostic tests do not provide information to distinguish the pulp inflammation stage while improvement of materials, techniques and vital pulp therapy have received a wider acceptance in symptomatic pulpitis treatment. The aim of this clinical study was to compare MMP-9 expression in healthy and inflamed pulp and to assess the short-term outcome of coronal pulpotomy in mature permanent teeth with symptomatic pulpitis.

Methods: Patients diagnosed with symptomatic pulpitis were included in this clinical study. Coronal pulpotomy was performed using calcium-silicate based material and blood samples were taken. Initial and postoperative pain was recorded by Heft-Parker visual analog scale at 24 hours and 72 hours after the procedure. In control group teeth with healthy pulp were used. During follow-up visits after three and six-months, patients were examined clinically and radiographically. The ELISA kit was used to determine the levels of MMP-9 in inflamed and healthy coronal pulp tissue.

Enrollment: 20 patients

Sex: All

Ages: 14 to 33 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Teeth diagnosed with symptomatic pulpitis
• Patients who did not use any NSAIDs before the treatment
• Positive cold test of investigated teeth
• Clinically dental caries in contact with pulp chamber
• Permanent teeth with radiographically closed root apex

Exclusion criteria

• Patients who refuse to participate in the study
• Medically compromised patients (with immunosuppressive/systemic diseases, patients on medications)
• Teeth with periapical radiolucency or clinical signs of apical periodontitis
• Periodontological compromised teeth (probing depth ≥4mm)
• Internal/external root resorption in periapical radiograph
• Pulp chamber and/or root canal calcification in periapical radiograph
• Teeth with unrestorable crown
• Teeth with a negative response to cold test

Trial design

Primary purpose: Treatment

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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20 participants in 2 patient groups

Control group - Healthy teeth

Other group

Description:

Patients with clinically healthy third molar or premolar teeth with indications for extraction; no clinical signs of pulpitis, no caries, no indications for the replacement of an old filling that is 1 mm from the pulp space as determined on the radiograph; a normal response to the cold test, and no apical radiolucency on the radiograph. For teeth of this group coronal pulpotomy was applied.

Treatment:

Procedure: Coronal pulpotomy

Test group - Teeth with pulpitis

Experimental group

Description:

Patients with symptomatic pulpitis resulting from caries; tooth pain defined as sharp, dull, localized or diffuse; pain at night; a symptomatic tooth with a positive response to the cold test and lingering pain; intermittent or continuous episodes of spontaneous pain (with no external stimulus) that could last from a few minutes up to a few hours; no apical radiolucency on the radiograph. For teeth of this group coronal pulpotomy was applied.

Treatment:

Procedure: Coronal pulpotomy