Modification of the Human Colon and Oral Microbiome by Allogenic HSCT

Federal Research Institute of Pediatric Hematology, Oncology and Immunology

Status: Unknown

Conditions: Human Microbiome; HSCT

Study type: Observational

Funder types: Other

Identifiers

NCT03942159

NCHPOI-2019-01

Details and patient eligibility

About

Allogenic HSCT brings significant changes in biodiversity and composition of the gut microbiome through antibiotic usage, the mucosal damage due to the chemo- and radiotherapy toxicity; compromised oral nutritional intake and graft-versus-host disease with gut damage as the complication. Aim of the study is to investigate the composition of the microbiota in both recipient and nursing relative donor, reveal changes in biodiversity after HSCT via 3-time points V3V4 16S rRNA and NGS sequencing of the colon and oral swabs, 3-indoxyl-sulfate measurement in the urine.

Enrollment: 15 estimated patients

Sex: All

Ages: Under 18 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• indications to alloHSCT
• nursing healthy donors 3-55 y.o.

Exclusion criteria

• unable to give samples for the test
• graft rejection
• previous HSCT

Trial design

15 participants in 2 patient groups

Donors

Description:

HLA-matched or haploidentical nursing relative donors

Patients

Description:

recipients of allogeneic HSCT