Modulation of Muscle Protein Metabolism by Essential Amino Acids (Aj1B)

University of Nottingham

Status: Unknown

Conditions: Old Age

Treatments: Dietary Supplement: 15g Mixed Essential Amino Acids; Dietary Supplement: 15g Mixed Essential Amino Acid REFEED; Dietary Supplement: 3g Leucine

Study type: Interventional

Funder types: Other; Industry

Identifiers

NCT01890369

B13122012 GEM BMS

Details and patient eligibility

About

The investigators plan to explore the ability of amino acids, the building blocks of proteins in our bodies and in the food we eat, to stimulate muscles to grow and stay strong. The investigators will investigate how rapidly after one meal the human body is ready to make use of a second meal and look at the molecular mechanisms underlying this. The investigators will look at these phenomena in older men as this group is known to be subject to a gradual loss of muscle mass and strength.

Full description

Muscles can stay very constant in size for much of adult life yet dramatic wasting is seen in advanced age and ill health. A better understanding of how muscles regulate their size will help design treatments to slow wasting in disease and to promote active aging. We plan to study a group of older men, ~70yrs, the age at which muscle bulk starts to decrease, to observe how the muscles respond to feeding. We will provide a drink that contains the digested products of proteins from which muscles are built. Taking blood samples and some tiny biopsies of muscle (done under local anaesthesia) will allow us to measure muscle turnover and we will be able to measure for how long after a meal muscles actively take in nutrients before becoming "full". We will also measure how long the muscle remains "full" before it returns to a state where it can again be stimulated to take in nutrients. We will also explore if leucine, one of the component parts of protein (especially in meat and dairy products) and a food widely used supplement, can promote the early re-stimulation of muscle by eating.

Enrollment: 32 estimated patients

Sex: Male

Ages: 65 to 75 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Male
• 65-75yrs
• BMI 18-28

Exclusion criteria

• Type 2 Diabetes mellitus
• Neurological Disease
• Significant cardiac, hepatic or renal disease
• Active malignancy
• Medication inc. Corticosteroids, NSAID use, Beta adrenoblockers.

Trial design

Primary purpose: Basic Science

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: Triple Blind

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32 participants in 4 patient groups

Early leucine refeed

Experimental group

Description:

15g Mixed Essential Amino Acids. 90 min delay. 3g Leucine

Treatment:

Dietary Supplement: 3g Leucine

Dietary Supplement: 15g Mixed Essential Amino Acids

Early EAA refeed

Experimental group

Description:

15g Mixed Essential Amino Acids. 90 min delay. 15g Mixed Essential Amino Acid REFEED

Treatment:

Dietary Supplement: 15g Mixed Essential Amino Acid REFEED

Dietary Supplement: 15g Mixed Essential Amino Acids

Mid latency EAA refeed

Experimental group

Description:

15g Mixed Essential Amino Acids. 150 min delay. 15g Mixed Essential Amino Acid REFEED

Treatment:

Dietary Supplement: 15g Mixed Essential Amino Acid REFEED

Dietary Supplement: 15g Mixed Essential Amino Acids

Late latency EAA refeed

Experimental group

Description:

15g Mixed Essential Amino Acids. 210 min delay. 15g Mixed Essential Amino Acid REFEED

Treatment:

Dietary Supplement: 15g Mixed Essential Amino Acid REFEED

Dietary Supplement: 15g Mixed Essential Amino Acids