Molecular Analysis in Peritoneal Washing Citology for Locally Advanced Gastric Cancer

Corporacion Parc Tauli

Status: Unknown

Conditions: Polymerase Chain Reaction; Gastric Cancer

Study type: Observational

Funder types: Other

Identifiers

NCT03098758

CIRPT_AL2017_1

Details and patient eligibility

About

Gastric carcinoma is a disease with a high mortality. Peritoneal washing cytology has a recognized prognostic utility. However sensibility of this study is not appropriate. Studies with PCR (polymerase chain retrotranscription) can increase sensibility and accurate staging information.

Objective: To analyze the sensibility and specificity of the expression of CK20, CEA and Ber-EP4 with PCR technique in peritoneal washings of advanced gastric carcinoma without clinical evidence of peritoneal carcinomatosis.

Methodology: Retrotranscription analysis (RT-PCR) and quantitative PCR (qPCR) of CK20, CEA and Ber-EP4 in peritoneal washing. Test the feasibility of qPCR with this new marker (Ber-EP4). Comparison of the results with data obtained with conventional cytology. Study of peritoneal recurrence.

Enrollment: 20 estimated patients

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patients with resectable advanced gastric cancer
• Informed consent

Exclusion criteria

• Macroscopic peritoneal carcinomatosis
• Non operable patients