Molecular Analysis of IgE Antibodies in Walnut Allergic Patients

University of Zurich (UZH)

Status

Completed

Conditions

Food Allergy

Treatments

Other: walnut allergy provocation

Study type

Interventional

Funder types

Other

Industry

Identifiers

NCT02295267

2012-0519

Details and patient eligibility

About

Accurate diagnosis of food allergy depends on the identification of the causative allergenic molecule(s). The proposed study intends to investigate and compare the pattern of IgE antibody sensitisation in walnut allergic subjects from three different regions of Europe (south (SEU) and central/north (C/NEU), to evaluate the diagnostic utility of a panel of novel allergen reagents.

Full description

Accurate diagnosis of food allergy depends on the identification of the causative allergenic molecule(s). Thus, full characterization of the major allergens of walnut and their clinical importance is an important undertaking.

The proposed study intends to investigate and compare the pattern of IgE antibody sensitisation in walnut allergic subjects from three different regions of Europe (south (SEU) and central/north (C/NEU), to evaluate the diagnostic utility of a panel of novel allergen reagents.

With a view towards improved in vitro diagnostic methods, we intend to evaluate the diagnostic potential of recombinant walnut allergens in patients with walnut allergy confirmed by a positive provocation or supported by a convincing history of anaphylactic reaction(s) to walnut in the past, patients with pollinosis but no symptoms of walnut allergy and non-atopic control subjects.

Enrollment

61 patients

Sex

All

Ages

1 to 70 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Signed informed consent
Group A: positive case history of allergic reaction(s) to walnut
Group B: positive case history of allergy to birch, grass or olive pollen and no case history of allergic reaction(s) to walnut
Group C: no case history of atopic disease

Exclusion criteria

Known pregnancy
Breast-feeding of infant
Treatment with the following drugs (or shortest interval between last treatment and food challenge or SPT):
corticosteroids (2 weeks): applied systemically, to the nose or locally on the skin test area.
antihistamines (3 days) except hydroxyzine (10 days)
betablocker agents (1 day)
angiotensin converting enzyme inhibitors (2 days)
Any major organic or infectious disease