Molecular and Hormonal Responses to Diet-Induced Insulin Resistance

Loughborough University

Status: Completed

Conditions: Healthy

Treatments: Dietary Supplement: High-fat, high-energy diet

Study type: Interventional

Funder types: Other

Identifiers

NCT03879187

R13-P171

Details and patient eligibility

About

This study determined the effect of 7 days of high-fat overfeeding on whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function in 15 healthy young individuals.

Full description

Fifteen healthy, young individuals underwent metabolic testing before and after 7 days of high-fat overfeeding. We determined whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function. Stable isotope tracers were used to assess the rate of appearance of glucose from the gut, liver glucose output and whole-body glucose disposal. Insulin signalling was determined by Western blotting of skeletal muscle tissue. Microvascular function was assessed through the use of quantitative immunofluorescence microscopy. These measurements were performed both postabsorptively and following the ingestion of 50 grams of glucose + 15 grams of protein.

Enrollment: 15 patients

Sex: All

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Aged between 18 and 40 years
• Physically active
• Non-smoker
• Not taking any medication known to interfere with the study
• No history of cardiovascular disease or diabetes
• Weight stable for the last 6 months

Exclusion criteria

• Outside of age range
• Sedentary
• Smoker
• Taking medication known to interfere with the study
• History of cardiovascular disease or diabetes
• Unstable weight within last 6 months

Trial design

Primary purpose: Basic Science

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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15 participants in 1 patient group

High-fat diet

Experimental group

Description:

Participants underwent a 7 day high-fat, high-energy diet intervention with metabolic measurements before and after

Treatment:

Dietary Supplement: High-fat, high-energy diet