Molecular Taxonomy of Surgically-harvested Ocular Tissues Defined by Single-cell Transcriptomics (Eyesinglecell)

Nanjing Medical University

Status: Unknown

Conditions: Proliferative Vitreoretinopathy; Glaucoma; Pterygium; Diabetic Retinopathy

Treatments: Procedure: ocular surgery

Study type: Interventional

Funder types: Other

Identifiers

NCT04682054

Eyesinglecell

Details and patient eligibility

About

The surgically-harvested eye tissue (ie. vitreous-retinal proliferative membrane, outflow pathway, retinas, and pterygium) is a complex tissue responsible for maintaining intraocular homeostasis or mediating ocular pathogenesis. Dysfunction of one or more resident cell types within the tissues results in different ocular disorder, leading to vision loss, or even blindness. In this study, we aim to use single-cell RNA sequencing to generate a comprehensive cell atlas of surgically-harvested eye tissues.

Enrollment: 80 estimated patients

Sex: All

Ages: 18 to 90 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

1. Patients with ocular diseases and require surgery intervention.
2. The surgery intervention remove pathological tissue during surgery.
3. Patients consent to the treatment and single-cell sequencing.

Exclusion criteria

1. Surgically removed eye tissue cannot meet with the quality control of single-cell sequencing (mainly the tissue weight and cell counts);

Trial design

Primary purpose: Basic Science

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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80 participants in 1 patient group

surgically-removed eye tissue

Experimental group

Treatment:

Procedure: ocular surgery