mRNA Expression in Lymphocytes of Glaucoma Patients

University Hospital Basel

Status

Completed

Conditions

Healthy Subjects

Glaucoma

Study type

Observational

Funder types

Other

Identifiers

NCT00327509

004-KAR-2004-001

Details and patient eligibility

About

The aim of the study is to compare messenger ribonucleic acid (mRNA) expression of various genes in lymphocytes between glaucoma patients and sex and age-matched healthy subjects. A secondary objective is to analyze the impact of different forms of glaucoma or of a vasospastic propensity on the findings.

Full description

Glaucoma is a leading cause of blindness world-wide. Chronic primary open-angle glaucoma is the most common form among Caucasian patients. The glaucoma patients will be divided into four groups: (1) high tension glaucoma, (2) normal tension glaucoma, (3) pseudoexfoliation glaucoma, and (4) juvenile glaucoma. Healthy subjects are separated into two age groups. Vasospastic propensity will be assessed with a questionnaire: patients and subjects answering yes to the questions: "do you have always cold hands, even during summer time?" and "do other people tell you that you have cold hands?" will be classified as vasospastic, and as normals if they deny a history of cold hands. Blood will be drawn from an arm vein, lymphocytes will be isolated and mRNA of following genes will be assessed in these lymphocytes:

Nuclear proteins: NF-kappa B, XPGC, P53, XIAP; Multi-drug resistance proteins: ABC 1, ABC 8, MDR 3; Adhesion protein: ICAM 1; Blood brain barrier breakdown protein: P2Y; Energy metabolism proteins: Adrenodoxin, Adrenodoxin-reductase, Cytochrome p450, Cytochrome-reductase, Alcohol-dehydrogenase; Tissue remodeling proteins: MMP 9, MMP 8, MMP 14, TIMP 1, TIMP 2, TIMP 3, TIMP 4.

Enrollment

60 patients

Sex

All

Ages

18 to 85 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Glaucoma patients

diagnosis of chronic glaucoma with typical glaucomatous disc and visual field damage
a present or past diurnal tension curve with an average intraocular pressure above 21 mmHg without treatment HTG)
a present or past diurnal tension curve with an average intraocular pressure below 21 mmHg without treatment (NTG)
a present or past diurnal tension curve with an average intraocular pressure above 21 mmHg without treatment and signs of pseudoexfoliation in the anterior segment (PEX)
a juvenile-onset open-angle glaucoma with a present or past diurnal tension curve with an average intraocular pressure above 21 mmHg without treatment (Juvenile Glaucoma)

Healthy subjects

no history of ocular disease
no history of systemic disease
no history of alcohol/drug abuse
normal blood pressure (100-140 / 60-90 mmHg)
best corrected visual acuity above 20/25 in both eyes
no pathological findings upon a slit-lamp examination and indirect fundoscopy
IOP < 20 mmHg in both eyes

Exclusion criteria

Ametropia > 3 dpt
Iridocorneal angle extremely narrow with complete or partial closure as determined by gonioscopy
Pigmentary dispersion
any abnormality which in the physician's view would prevent reliable applanation tonometry
History of chronic or recurrent severe inflammatory eye disease such as scleritis or uveitis
History of ocular trauma or intraocular surgery within the past 6 months
History of infection or inflammation within the past 3 months
History of clinically significant or progressive retinal disease such as retinal degeneration, diabetic retinopathy or retinal detachment
Need for any concomitant medications that may interfere with the evaluation of ocular blood flow
significant history and/or active alcohol or drug abuse