mTOR and Adipose Tissue Inflammation

University Hospital Basel

Status: Completed

Conditions: Obesity

Treatments: Other: Adipose tissue sampling

Study type: Observational

Funder types: Other

Identifiers

NCT02881697

mTOR

Details and patient eligibility

About

The target of rapamycin complex 2 (TORC2) is an evolutionarily conserved serine/threonine protein kinase that controls growth and metabolism. In mammals (including humans), mammalian TOR complex 2(mTORC2) contains mammalian TOR (mTOR), RICTOR, mSIN1 protein, and mLST8 gene. In an animal model, the adipose-specific rictor knockout (AdRiKO) mouse, systemic insulin resistance, hepatic steatosis, and cardiovascular dysfunction develop upon high fat diet (HFD)-induced obesity or aging. To find a molecular link between adipose mTORC2 and systemic insulin resistance, investigators have already performed transcriptomics and proteomics analysis on visceral white adipose tissue in a mouse model. The aim of the study is to confirm a molecular link between adipose mTORC2 and systemic insulin resistance in humans.

Enrollment: 80 patients

Sex: All

Ages: 18 to 60 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Obese volunteers (BMI > 35kg/m2) aged 18 to a maximum of 60 years, males and females; insulin-resistant, scheduled for elective bariatric surgery.
• Normal weight patients (BMI < 27kg/m2) aged 18- max. 60 years, males and females; insulin-sensitive, scheduled for elective surgery.

Exclusion criteria

• Known diabetes mellitus
• Chronic inflammatory disease (inflammatory bowel disease, rheumatoid disease, cancer)
• Acute inflammatory disease
• Known renal disease: kidney failure
• Pregnancy:
• history of gastrointestinal surgery with major changes to the gastrointestinal tract (removal of stomach, any GI-bypass)
• Substance abuse, alcohol abuse
• Inability to follow procedures due to psychological disorders, dementia
• insufficient knowledge of project language (German)

Trial design

80 participants in 3 patient groups

Obese insulin-resistant subjects

Description:

Adipose tissue sampling in obese volunteers (BMI \> 35kg/m2) aged 18- max. 60 years, males and females; insulin-resistant, scheduled for elective bariatric surgery

Treatment:

Other: Adipose tissue sampling

Lean insulin-sensitive controls

Description:

Adipose tissue sampling in normal weight patients (BMI \< 27kg/m2) aged 18- max. 60 years, males and females; insulin-sensitive, scheduled for elective surgery

Treatment:

Other: Adipose tissue sampling

Obese diabetic subjects

Description:

Adipose tissue sampling in obese volunteers (BMI \> 35kg/m2) aged 18- max. 60 years, males and females; diabetic, scheduled for elective bariatric surgery

Treatment:

Other: Adipose tissue sampling