Multiparametric Assessment of Peripheral Blood and Tissue Myeloid Cells. Phenotype for Precision Medicine in Patients With SARS-Cov-2 Infection

Institute of Hospitalization and Scientific Care (IRCCS)

Status: Enrolling

Conditions: COVID-19 Pneumonia

Treatments: Other: Immune-phenotyping of tissue and peripheral blood myeloid compartment

Study type: Observational

Funder types: Other

Identifiers

NCT05767853

3213

Details and patient eligibility

About

We hypothesise that patients with SARS-Cov-2 infection are characterized by progressive changes in distribution of distinct lung macrophages populations mediated by influx of circulating monocytes into the lungs . Moreover, we also hypothesise that patients with higher rate of MerTKpos alveolar macrophages in the lung lavage will have the lowest rate of lung complications and the best recovery outcome in terms of clinical outcome and need of assisted ventilation supporting the use of macrophage phenotyping as novel prognostic biomarker in patients with SARS-Cov-2 infection. Finally, the definition of the transcriptomic signature of peripheral blood and tissue-derived myeloid cell subtypes will offer new therapeutic target of this uncurable newly discovered infection.

Enrollment: 30 estimated patients

Sex: All

Ages: 18 to 80 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patients with COVID-19 pneumonia

Exclusion criteria

• Other infections rather than Covid-19
• Patients with concomitant treatment with prednisone ≥10 mg daily
• Patients under therapy with immunosuppressants

Trial design

30 participants in 2 patient groups

SARS-Cov-2pos pneumonia with moderate/severe lung manifestations with clinical worsening

Description:

(PaO2/FiO2\<200)

Treatment:

Other: Immune-phenotyping of tissue and peripheral blood myeloid compartment

SARS-Cov-2pos pneumonia with moderate/severe lung manifestations with clinical improvement

Description:

(PaO2/FiO2\>200)

Treatment:

Other: Immune-phenotyping of tissue and peripheral blood myeloid compartment