Myeloid Derived Suppressor Cells Control by Signal Regulatory Protein-alpha: Investigation in Hepatocellular Carcinoma (MDScan)

Nantes University Hospital (NUH)

Status: Completed

Conditions: Hepatocellular Carcinoma

Treatments: Procedure: Collection of human samples

Study type: Observational

Funder types: Other

Identifiers

NCT02868255

RC16_0156

Details and patient eligibility

About

project is to study and develop anti-Signal Regulatory Protein α (SIRPα) antibodies (Ab) as a new immunotherapy strategy in cancer.

Samples harvested from hepatocellular carcinoma (HCC) and ovarian cancer patients will be used in evaluation of the SIRP-CD47 expression and of the effect of the anti-human Signal Regulatory Protein (hSIRP) Ab on various cellular types from patients and healthy volunteers.

Enrollment: 30 patients

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• adult
• HCC patients (all BCLC stages accepted)
• patient consent for the use of their biological samples

Exclusion criteria

• patient with other cancers
• hepatitis C or B positive
• HCC treatment by chemo-embolization under 3 months
• under Sorafenib treatment within the month prior to collection
• underage or under guardianship patients

Trial design

30 participants in 3 patient groups

Ascites

Description:

30 inflammatory ascites of HCC patients (Collection of human samples ) Ascites will be selected only from HCC patients with an elevated protein level (ie inflammatory ascites) Puncture of ascites will be collected by paracentesis on HCC or ovarian cancer patients during their routine care These biological samples are affiliated with the biobank "hépato-gastroentérologie du CHU de Nantes" (declared under the reference DC-2011-1399).

Treatment:

Procedure: Collection of human samples

Resections

Description:

30 HCC resections (Collection of human samples ) Fragment of resected HCC will be obtained after surgery and histological analysis will be performed by the anatomo-pathology service These biological samples are affiliated with the biobank "hépato-gastroentérologie du CHU de Nantes" (declared under the reference DC-2011-1399).

Treatment:

Procedure: Collection of human samples

blood samples

Description:

blood samples (Collection of human samples )will be collected prospectively for complementary functional analysis of peripheral These biological samples are affiliated with the biocollection "hépato-gastroentérologie du CHU de Nantes" (declared under the reference DC-2011-1399).

Treatment:

Procedure: Collection of human samples