Myofibroblastic Transformation Secondary to Epithelial-stromal Interactions in the Keratoconus (MYKE)

Fondation Ophtalmologique Adolphe de Rothschild

Status: Withdrawn

Conditions: Keratoconus

Treatments: Procedure: Corneal sampling

Study type: Observational

Funder types: NETWORK

Identifiers

NCT03990740

EGN_2017_22

Details and patient eligibility

About

Keratoconus is characterized by a thinning of the cornea, which causes a decrease in visual acuity due to astigmatism.

Publications suggest that keratoconus is linked to chronic inflammation (increase in pro-inflammatory cytokines and metalloproteinases (MMP). Direct epithelial-stromal interactions (D-ESI) have a role in the induction of metalloproteinases (MMP) and the differentiation of fibroblasts into myofibroblasts via an EMMPRIN membrane glycoprotein (extracellular matrix membran MMP inducer - CD 147). On a healthy cornea, EMMPRIN's effects are prevented by a lack of contact between epithelial and stromal cells through a basement membrane, which is altered in the keratoconus The hypothesis is that stromal thinning of the keratoconus could be related to increased expression of EMMPRIN by epithelial and stromal cells (resulting in increased MMP synthesis), with a preponderance at the most deformed areas.

The main objective is to demonstrate a transformation of fibroblasts to myofibroblasts in the corneal stroma of keratoconus patients.

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• For the cases :

  • Suffering from keratoconus and requiring a first optical corneal transplant

• For the controls:

  • Orbital exenteration operation due to an orbital tumor
  • Absence of any anomaly of the ocular surface observed during the slit lamp examination at the last preoperative consultation

Exclusion criteria

• For the cases:

  • Keratoconus patient requiring a tectonic corneal transplant
  • Known pregnancy, or breastfeeding

• For the controls:

  • History of orbital radiotherapy
  • History of corneal surgery
  • History of corneal surface tumour
  • Eye surface abnormality noted in the preoperative period
  • Known keratoconus
  • Known pregnancy, or breastfeeding
  • Secondary exclusion if a keratoconus is diagnosed during the immunohistochemical analysis by visualization of Bowman membrane interruption

Trial design

0 participants in 2 patient groups

Cases

Description:

Patients suffering from keratoconus and requiring a first optical corneal transplant

Treatment:

Procedure: Corneal sampling

Controls

Description:

Patients with an indication of orbital exenteration operation due to an orbital tumor

Treatment:

Procedure: Corneal sampling