MyoGene: Effects of Acute Exercise on (Myokine) Gene Expression in Human Skeletal Muscle

Wageningen University

Status

Completed

Conditions

Myokine

Muscle

Gene Expression

Exercise

Treatments

Other: Single legged exercise

Study type

Interventional

Funder types

Other

Identifiers

NCT01316731

NL33968.081.10

Details and patient eligibility

About

Rationale: Proteins released from muscle during and shortly after exercise, often referred to as myokines, may be central to our understanding of the cross-talk during and after exercise between skeletal muscles and other organs, in particular the liver. So far only a few myokines are identified (e.g. IL-6, IL-8, IL-15, TNF-alpha). Taking into account the role of these several known myokines in developing insulin resistance, revealing new putative myokines might provide valuable information and a direction for future research on the pathogenesis and treatment of type 2 diabetes mellitus.

Objective: The objective of the present study is to identify novel myokines, expression of which is altered in skeletal muscle after a single bout of exercise.

Study design: experimental study. Study population: Ten healthy, male subjects between 40 and 60 years of age and BMI < 30 kg/m2, will participate in this study.

Intervention: A single exercise bout that consists of one hour one-legged cycling on a adapted recumbent cycle ergometer at a submaximal rate. The non-exercising leg will serve as control for the exercising leg.

Main study outcomes: Main study outcomes include upregulation of genes in skeletal muscle after exercise (with a focus on genes encoding myokines) and changes of blood plasma levels of selected proteins after exercise.

Enrollment

12 patients

Sex

Male

Ages

40 to 60 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Age 40-60 years
Male gender
BMI < 30 kg/m2

Exclusion criteria

Exercising regularly (> 2 times a week, > 3 hour in total per week)
Unstable body weight (weight gain or loss > 3 kg in the past three months)
Following, or have recently followed a (weight-loss) diet
Donated or intended to donate blood 2 months before until two months after the study
Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease)
Systolic blood pressure >160 mmHg and/or diastolic blood pressure >100 mmHg
Use of medications known to interfere with gene expression in the muscles (i.e. statins, fenofibrate).
Use of antithrombotic therapy (marcoumar, sintromitis).
Diagnosed diabetes mellitus type 1 or 2.
Drugs or alcohol abuse ( > 3 glasses of alcoholic beverages a day).
(Chronic) injuries of the locomotor system that can interfere with the intervention
Participated in another study within the last six months