Nasal Epithelium Gene Expression Profiling in Child Respiratory Allergic Disease

Centre Hospitalier Universitaire de Nice

Status: Completed

Conditions: Allergic Rhinitis

Treatments: Procedure: Collection of nasal epithelial cells

Study type: Interventional

Funder types: Other

Identifiers

NCT00569361

CIR 2005

Details and patient eligibility

About

Using a human pangenomic microarray, the researchers established expression profiles of nasal epithelial cells, collected by brushing of patients belonging to one of four distinct groups:

1. allergic rhinitis to dust mite (AR) isolated (n=12),
2. AR associated with bronchial hyperreactivity (n=12),
3. AR associated with asthma (n=14),
4. control (n=14).

Full description

Context: Asthma is the most frequent chronic disease in childhood. Allergic rhinitis has been described as a risk factor to develop asthma. The objective of the study was to evaluate the contribution of the respiratory epithelium to development of allergic rhinitis and asthma and to identify the molecular mechanisms driving rhinitis toward asthma.

Methods: Using a human pangenomic microarray, we established expression profiles of nasal epithelial cells, collected by brushing of patients belonging to one of four distinct children 2 to 18 years of age groups:

1. allergic rhinitis to dust mite (AR) isolated (n=12),
2. AR associated with bronchial hyperreactivity (n=12),
3. AR associated with asthma (n=14),
4. control (n=14).

Enrollment: 80 patients

Sex: All

Ages: 6 to 17 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

Patients:

• Patients between 6 and 17 years old
• Patients with allergic rhinitis

Witness:

• Patients between 6 and 17 years old
• Patients without allergic rhinitis and asthma

Exclusion criteria

• Rhino-bronchitis infection dated from less than 15 days
• Patients younger than 6 or older than 17 years

Trial design

Primary purpose: Screening

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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80 participants in 1 patient group

A

Other group

Description:

Collection of nasal epithelial cells by brushing

Treatment:

Procedure: Collection of nasal epithelial cells