Nd:YAG Laser Effects on Subgingival Microbiota in Stage III-IV Periodontitis

Rui He

Status: Active, not recruiting

Conditions: Periodontitis

Treatments: Procedure: ND; Procedure: SRP

Study type: Interventional

Funder types: Other

Identifiers

NCT07061756

[2024]493-1

Details and patient eligibility

About

This randomized controlled trial (RCT) aims to evaluate the clinical efficacy of Nd:YAG laser adjunctive therapy and its impact on the subgingival microbiome in patients with stage III-IV periodontitis. The study addresses two key questions: (1) Whether Nd:YAG laser combined with scaling and root planing (SRP) yields significantly greater improvements in periodontal parameters compared to SRP alone; and (2) How subgingival microbial dynamics correlate with therapeutic outcomes.

Participants were randomized into two groups: the SRP group (mechanical debridement only) and the Nd group (mechanical debridement with Nd:YAG laser therapy). Clinical parameters-including probing depth (PD), clinical attachment level (CAL), and gingival index (GI)-were assessed at baseline and 3 months post-treatment. Subgingival plaque samples were analyzed via 16S rDNA sequencing to characterize microbial community shifts.

Enrollment: 240 estimated patients

Sex: All

Ages: 18 to 80 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Aged 18-80 years
• Minimum of 14 remaining natural teeth
• Diagnosis of Stage III-IV periodontitis according to the 2018 World Workshop classification system
• No periodontal treatment within the past 6 months
• Willing to provide informed consent and participate in the study protocol

Exclusion criteria

• Presence of systemic conditions that may affect periodontal treatment outcomes (e.g., uncontrolled hypertension [>140/90 mmHg], diabetes mellitus [HbA1c >7%])
• Use of antibiotics or medications known to induce gingival enlargement (e.g., calcium channel blockers, phenytoin) within the past 3 months
• Coagulation disorders or hematologic diseases
• Active systemic infections
• Pregnancy or lactation
• Acute periodontal lesions
• Inability to comply with study protocols

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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240 participants in 2 patient groups

Nd group

Experimental group

Description:

The Nd group received comprehensive periodontal therapy combining scaling and root planing (SRP) with adjunctive Nd:YAG laser treatment.

Treatment:

Procedure: ND

SRP group

Active Comparator group

Description:

The SRP group received standard periodontal therapy consisting of scaling and root planing (SRP) alone, serving as the active control for comparison against laser-assisted treatment.

Treatment:

Procedure: SRP