Nelfinavir for the Treatment of Gammaherpesvirus-Related Tumors

Johns Hopkins Medicine

Status and phase

Terminated

Early Phase 1

Conditions

Hodgkin Lymphoma

EBV

Nasopharyngeal Cancer

Gastric Cancer

Castleman Disease

Kaposi Sarcoma

Non-Hodgkin Lymphoma

Treatments

Drug: Nelfinavir

Study type

Interventional

Funder types

Other

Identifiers

NCT02080416

J13174

NA_00092477 (Other Identifier)

Details and patient eligibility

About

The goals of this study is to determine if nelfinavir can target Epstein-Barr virus (EBV) and Kaposi sarcoma-associated herpesvirus (KSHV) in patients with certain cancers.

Full description

Epstein-Barr virus (EBV) and Kaposi sarcoma-associated herpesvirus (KSHV) are gammaherpesviruses that are associated with a variety of human cancers, including a subset of lymphomas, carcinomas, and sarcomas. In tumors the virus typically exists in a latent state. In latently infected cells, the vast majority of viral genes are not expressed and there is little to no production of infectious virions. The virus replicates in tandem with cell division using cellular machinery. This highly restricted pattern of gene expression allows the virus to evade immune recognition and clearance.

Currently, the treatment approach to virally-associated malignancies is no different than the treatment approach to the same tumors where there is no viral association. Yet, the presence of virus within these tumors offers an opportunity to develop virus-specific, targeted therapies in these diseases. Such therapies might not only be more effective but also less toxic. EBV- and KSHV-associated cancers are more common in patients with HIV, congenital immunodeficiencies, or other immunosuppression, such as transplant recipients. These patients in particular would benefit from more targeted treatment approaches to their malignancies, potentially sparing the toxicities of cytotoxic chemotherapy in an already immunocompromised patient population.

Activation of lytic gene expression in virally-infected tumors may enhance tumor-specific cell killing through multiple mechanisms. Importantly, the cytotoxic effects of antiviral nucleoside analogues, such as acyclovir and its cogeners, depend on the activity of viral kinases which are only expressed during lytic replication. Because EBV(+) or KSHV(+) tumors are characterized by latent viral infection, these antiviral drugs as a single agent are not active in these tumors. However, if lytic gene expression could be activated in virally-associated tumors, this could render EBV(+) and KSHV(+) tumor cells susceptible to killing by antiviral nucleoside analogues.

Nelfinavir (NFV), an FDA-approved protease inhibitor for the treatment of HIV, has been shown to be a potent activator of lytic gene expression of EBV(+) and KSHV(+) cancer cell lines. Furthermore, NFV is able to activate lytic gene expression of EBV and KSHV at drug levels that are achievable in humans. There is also growing evidence that NFV has antitumor activity.

The goals of this study is to determine if NFV activates lytic gene expression in the tumors and causes tumor regression in patients with EBV(+) or KSHV(+) cancers.

Enrollment

1 patient

Sex

All

Ages

18+ years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Age 18 years or older
Biopsy proven EBV(+) or KSHV(+) malignancy
Relapsed/refractory disease failing > 2 prior therapies
Measurable, non-bony disease (at least one lesion on radiographic or physical exam assessment measuring > 2 cm in longest axis)
KS patients with skin-only disease must have cutaneous lesions amenable to four 3 mm punch biopsies during the course of the study
Eastern Cooperative Oncology Group (ECOG) performance status < 2
Life expectancy of greater than 12 weeks
Patients must be able to lie flat for at least 60 minutes and fit on a PET-CT scanner
Ability to comply with an oral drug regimen
Females of childbearing potential must have a negative pregnancy test at screening
Patients must have normal organ and marrow function as defined below within 14 days of study entry

Exclusion criteria

Patients with HIV-associated primary central nervous system lymphoma
Radiotherapy or chemotherapy ending within 14 days of study enrollment
Patients currently on other protease inhibitors
Chronic diarrhea
Acute, active infection within 14 days of enrollment
Patients on active treatment for hypo- or hyperthyroidism
End-stage liver disease unrelated to tumor
Hepatitis B or hepatitis C infection
Use of any other type of investigational agent or treatment concurrently or within 28 days before the first dose of study treatment
History of iodine hypersensitivity
Females who are pregnant or breastfeeding
Physical or psychiatric conditions that in the estimation of the investigator place the patient at high risk of toxicity, non-compliance, or inability to complete the study requirements
Use of drugs to treat or prevent herpesvirus infections
Essential medication that is known to interact with nelfinavir