Neuropeptide Y Regulates Neurogenic Pulp Inflammation

Institucion Universitaria Colegios de Colombia

Status: Completed

Conditions: Dental Pulp Disease; Neurogenic Inflammation

Treatments: Other: H2O2 at 25% plus cold blue light for 15 minutes; Other: H2O2 at 35% for 8 minutes; Other: H2O2 at 40% for 20 minutes

Study type: Interventional

Funder types: Other

Identifiers

NCT06606236

BIO407

Details and patient eligibility

About

Objective. This study assessed the neuropeptide Y (NPY) expression in healthy human dental pulp following tooth bleaching with three in-office hydrogen peroxide-based systems: Opalescence Boost© (Ultradent Products, South Jordan, UT), Pola Office© (SDI, Victoria, Australia), and Zoom© (Zoom! Bleaching System; Discuss Dental, Culver City, CA).

Materials and Methods. Forty dental pulps were collected from healthy premolars scheduled for extraction for orthodontic reasons. Teeth were divided into four groups containing ten healthy premolars each: Control group (n= 10): the teeth were not exposed to dental bleaching agents (healthy pulps assessed for normal/basal NPY values). Pola Office system group (n= 10): application of Pola office (35% H2O2) for 8 minutes. Opalescent Boost system group (n= 10): application of Opalescent Boost (40% H2O2) for 20 minutes. Zoom system group (n= 10): application of Zoom! (25% H2O2 + cold blue light) for 15 minutes. The investigators rigorously followed the manufacturer's instructions for all bleaching systems. Following the extractions, the pulpal tissue was collected, placed in a 4% formaldehyde solution in Eppendorf tubes, and processed. NPY levels were measured using enzyme-linked immunosorbent assay (ELISA).

Enrollment: 40 patients

Sex: All

Ages: 18 to 27 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Dental pulps were collected from non-smoking donors
• Patients who need premolar extractions for orthodontic purposes.
• These premolars must meet the following requirements:
• Caries and restoration-free,
• Complete root development (visually and radiographically confirmed),
• Normal response to sensitivity testing.

Exclusion criteria

• Evidence of periodontal disease,
• Traumatic occlusion
• Previous orthodontic force application.

Trial design

Primary purpose: Diagnostic

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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40 participants in 4 patient groups

Control group

No Intervention group

Description:

The teeth were not exposed to dental bleaching agents (healthy pulps with normal/basal NPY values). n=10

Pola Office system group

Experimental group

Description:

Application of Pola office n=10

Treatment:

Other: H2O2 at 35% for 8 minutes

Opalescent Boost system group

Experimental group

Description:

Application of Opalescent Boost n=10

Treatment:

Other: H2O2 at 40% for 20 minutes

Zoom system group

Experimental group

Description:

Application of Zoom! n=10

Treatment:

Other: H2O2 at 25% plus cold blue light for 15 minutes