Nitrate Supplementation; Source (NO-what)

Maastricht University Medical Centre (MUMC)

Status and phase: Completed; Phase 4

Conditions: Nutritional Physiological Phenomena; Blood Pressure

Treatments: Dietary Supplement: Spinach; Dietary Supplement: Rocket salad; Dietary Supplement: Sodium nitrate; Dietary Supplement: Beetroot juice

Study type: Interventional

Funder types: Other

Identifiers

NCT02271633

METC14-3-020

Details and patient eligibility

About

The main aim of the current study will be to compare the effects of ingesting different nitrate-rich sources on plasma nitrite concentrations.

Full description

Oral ingestion of nitrate (NO3-) has been shown to increase plasma nitrate and nitrite levels. Significant lowering of pulmonary oxygen uptake during exercise, as well as significantly lower blood pressure values at rest have been observed after supplementation with nitrate. However, no study has yet described a direct comparison between different nitrate-rich beverages with respect to the bioavailability of nitrate/nitrite and the possible hemodynamic effects. Based on the gaps in current literature, our main goal will therefore be to gain further insight into the effects of nitrate supplementation with different sources on plasma nitrite levels.

Enrollment: 30 patients

Sex: All

Ages: 18 to 45 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• 18.5 < BMI < 30 kg/m2
• Engagement in regular physical activity ( > 1 h a week)

Exclusion criteria

• Use of medication
• Smoking
• Currently supplementing diet with nitrate
• Lactose intolerance

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: None (Open label)

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30 participants in 4 patient groups

Sodium nitrate

Active Comparator group

Description:

Dietary supplement: 800 mg of nitrate in sodium nitrate added with water to get a 140 mL solution (BASF, Ludwigshafen, Germany)

Treatment:

Dietary Supplement: Beetroot juice

Dietary Supplement: Spinach

Dietary Supplement: Rocket salad

Beetroot juice

Active Comparator group

Description:

Dietary supplement: 800 mg of nitrate in concentrated beetroot juice (Beet-IT)

Treatment:

Dietary Supplement: Sodium nitrate

Dietary Supplement: Spinach

Dietary Supplement: Rocket salad

Spinach

Active Comparator group

Description:

Dietary supplement: 800 mg of nitrate in a spinach based beverage

Treatment:

Dietary Supplement: Beetroot juice

Dietary Supplement: Sodium nitrate

Dietary Supplement: Rocket salad

Rocket salad

Active Comparator group

Description:

Dietary supplement: 800 mg of nitrate in a rocket salad based beverage

Treatment:

Dietary Supplement: Beetroot juice

Dietary Supplement: Sodium nitrate

Dietary Supplement: Spinach