(NOD)-Like Receptors CARD Domain-Containing 4 Levels in Individuals With Periodontitis

Saglik Bilimleri Universitesi

Status: Unknown

Conditions: Periodontitis; Healthy; Gingivitis

Treatments: Other: Saliva; Other: gingival crevicular fluid

Study type: Observational

Funder types: Other

Identifiers

NCT04850768

21-29

Details and patient eligibility

About

NLRC4, an inflammasome , is a cytosolic multiprotein complex involved in the initiation and modulation of the immune response. Periodontitis is a chronic inflammatory disease that can start with localized inflammatory reactions created by the supporting tissues surrounding the teeth against microorganisms and then result in loss of teeth. It has been said that proinflammatory cytokines released in the microenvironment of periodontitis can increase the expression of NLRC4 inflammasome genes. The authors think that NLRC4 may play a role in the periodontitis. The aim of this study is to compare the NLRC4, IL-1β and IL-10 levels of healthy and periodontitis individuals.

Full description

Periodontitis is a destructive chronic inflammatory disease that can start with localized inflammatory reactions created by the supporting tissues surrounding the teeth against microorganisms and then result in loss of teeth. Inflammasomes are cytosolic multiprotein complex involved in the initiation and modulation of the immune response.

Interleukine -1β ( IL-1β ) levels are associated with periodontal destruction in the pathogenesis of periodontal disease. Interleukine-10 (IL-10) has been reported to decrease periodontal disease progression rate.

Gingival crevicular fluid shows the cellular response in the periodontium. The most important host-related ingredients are inflammatory markers, including cytokines, enzymes, and interleukins. Saliva analysis is used in the diagnosis of systemic conditions as well as in the diagnosis of oral pathologies.

Both NLRC4 and NLRP3 inflammasomes have been shown to play an important role in the induction of interleukin-1β secretion. Periodontitis is induced by a complex microbial biofilm containing multiple Gram-positive and Gram-negative bacteria (including flagellate microorganisms) capable of activating a large number of NLRs. It has been said that proinflammatory cytokines released in the microenvironment of periodontitis can increase the expression of NLRC4 inflammasome genes. Evidence on the role of inflammasomes in periodontal disease is still very poor. The aim of this study is to compare the NLRC4, IL-1β and IL-10 levels of healthy and periodontitis individuals.

Enrollment: 75 estimated patients

Sex: All

Ages: 18 to 70 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• systemically healthy individuals who does not have a disease under treatment, individuals without regular systemic medication

Exclusion criteria

• smoking receiving periodontal treatment in the last 6 months pregnancy menopausal or menstrual period

Trial design

75 participants in 3 patient groups

Periodontitis

Description:

Individuals with Periodontitis

Treatment:

Other: gingival crevicular fluid

Other: Saliva

Gingivitis

Description:

Individuals with Gingival Inflammation

Treatment:

Other: gingival crevicular fluid

Other: Saliva

Healthy

Description:

Individuals with Periodontally Healthy

Treatment:

Other: gingival crevicular fluid

Other: Saliva