Non Carbonic Buffer Power of Critical Ill Patients With Sepsis

Institute of Hospitalization and Scientific Care (IRCCS)

Status: Completed

Conditions: Acid-Base Imbalance; Respiratory Alkalosis; Respiratory Acidosis

Treatments: Diagnostic Test: In vitro determination of non-carbonic buffer power; Diagnostic Test: Classic description of acid-base status

Study type: Observational

Funder types: Other

Identifiers

NCT03503214

Buffer power

Details and patient eligibility

About

Alterations of acid-base equilibrium are very common in critically ill patients and understanding their pathophysiology can be important to improve clinical treatment.

The human organism is protected against acid-base disorders by several compensatory mechanisms that minimize pH variations in case of blood variations in carbon dioxide content. The aim of the present study is to quantify the buffer power, i.e. the capacity to limit pH variations in response to carbon dioxide changes, in critically ill septic patients and compare these results with data collected from healthy volunteers.

Full description

Alterations of acid-base equilibrium are very common in critically ill patients and understanding their pathophysiology can be important to improve clinical treatment.

The human body is protected against acid-base disorders by several compensatory mechanisms that minimize pH variations in response to acid-base derangements.

The present study focuses on the acute compensatory mechanisms of respiratory acid-base derangements, i.e., respiratory acidosis and respiratory alkalosis. In this case the non-carbonic buffers are constituted by albumin and phosphates in plasma, with the addition of hemoglobin in whole blood.

Aim of the present in-vitro study is to measure the buffer power of non-carbonic weak acids contained in whole blood and isolated plasma, assess the relative contribution of red blood cells and plasma proteins and perform a comparison between septic patients and healthy controls.

Enrollment: 36 patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Septic patients and healthy volunteers

Exclusion criteria

• age < 18 years and pregnancy

Trial design

36 participants in 2 patient groups

Septic patients

Description:

Patients with sepsis or septic shock according to the SEPSIS-III (Singer M Jama 2016) admitted to the general Intensive Care Unit

Treatment:

Diagnostic Test: In vitro determination of non-carbonic buffer power

Diagnostic Test: Classic description of acid-base status

Healthy volunteers

Description:

Subjects without known respiratory, cardiovascular, hepatic, renal or hematologic diseases.

Treatment:

Diagnostic Test: In vitro determination of non-carbonic buffer power

Diagnostic Test: Classic description of acid-base status