Novel Approach With Double Agent Artificial Oocyte Activation for Repeated Fertilization Failure Due to Combined Sperm and Oocyte Factor (CISC)

Ibn Sina Hospital

Status: Completed

Conditions: Infertility

Treatments: Other: Oocyte double chemical activation

Study type: Interventional

Funder types: Other

Identifiers

NCT02418416

IbnSina Hospital IVF

Details and patient eligibility

About

Novel approach for Oocyte fertilization failure for difficult cases not respond to traditional methods of assisted reproduction as intracytoplasmic sperm injection.

Full description

In some cases undergoes assisted reproduction technique specially Intracytoplasmic sperm injection, in subgroup of patient they fail to fertilize or poorly fertilized, some authors recommend chemical activation for those group of patient with Ca ionophore but still with Ca ionophore there are some cases with total fertilization failure or low fertilization specially in those patient with 2 separate factors like teratozoospermia with poor quality Oocytes so the investigators decided to test the effect of double activation methods by using tow agents proven with life birth rate Ca ionophore and strontium chloride.

We Do intracytoplasmic sperm injection and immediately activate oocyte with Ca Ionophore followed by strontium chloride activation and monitoring the embryogenesis until the blastocyst formation and pregnancy outcome in multicenter assessment.

Enrollment: 400 patients

Sex: Female

Ages: 18 to 44 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• poor sperm
• poor quality Oocytes
• previous failed fertilization
• previous poor embryogenesis

Exclusion criteria

• normal patient
• previous successful outcome

Trial design

Primary purpose: Supportive Care

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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400 participants in 2 patient groups

Double activation

Experimental group

Description:

The Oocytes in this arm will undergo double activation with Ca ionophore 5 micro mol for 20 min them Strontium chloride 10 micro mol for 60 min.

Treatment:

Other: Oocyte double chemical activation

Control arm

No Intervention group

Description:

The Oocytes will undergo Intracytoplasmic sperm injection only