Nutritional Strategies to Augment the Postprandial Muscle Protein Synthetic Response to the Ingestion of a Low Dose of Protein in Middle-aged Women

University of Illinois at Urbana-Champaign

Status: Completed

Conditions: Aging

Treatments: Dietary Supplement: 6.6 g Whey protein + 1.25 g leucine + 0.8 g Citrulline; Behavioral: Resistance Exercise; Dietary Supplement: 6.6 g Whey protein + 1.25 g leucine; Dietary Supplement: 14 g Whey protein; Other: Water control; Dietary Supplement: 4 g Whey protein + 1.25 g leucine + 2.6 g Whey peptides

Study type: Interventional

Funder types: Other

Identifiers

NCT02918981

16999

Details and patient eligibility

About

Using stable isotope methodology, investigators will determine the postabsorptive and postprandial muscle protein synthetic response in 60 female adults (Age 50-79, BMI: <30 kg/m2) immediately after an acute bout of resistance exercise. Participants will be divided in to 5 groups of twelve women (n=12). Each group will receive a different protein beverage or placebo to consume following the resistance exercise training. Group 1 will consume a beverage consisting of whey protein; group 2 will consume whey protein supplemented with additional leucine; group 3 will consume whey protein supplemented with additional leucine and hydrolyzed whey peptides; group 4 will consume whey protein supplemented with additional leucine and citrulline; group 5 will just consume water. During the testing, blood and muscle samples will be collected.

Enrollment: 50 patients

Sex: Female

Ages: 20 to 79 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• BMI <30 kg/m2
• Healthy, sedentary
• Either 20-30 years old or 50-79 years old

Exclusion criteria

• Tobacco use
• Allergies to milk consumption
• Unusually high protein consumption
• Vegan diet
• Allergies to antibiotics or xylocaine
• Phenylketonuria (PKU)
• Diagnosed GI tract diseases
• Arthritic conditions
• A history of neuromuscular problems
• Heart disease
• Metabolic disorders
• -Liver, kidney, or urinary disease
• Musculoskeletal problems
• Autoimmune disease
• Neurological disease
• Previous participation in amino acid tracer studies
• Predisposition to hypertrophic scarring or keloid formation
• Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
• Individuals using thyroid medications or other medications affecting endocrine function
• Pregnancy
• Contraindications for exercise
• BMI ≥30
• supplements that influence protein metabolism (e.g omega 3 fish oils)

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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50 participants in 5 patient groups

Whey protein

Active Comparator group

Description:

After resistance exercise, participants aged 50-79 years will consume 14g Whey protein dissolved in 200 mL of water

Treatment:

Behavioral: Resistance Exercise

Dietary Supplement: 14 g Whey protein

Whey + Leucine

Experimental group

Description:

After resistance exercise, participants aged 50-79 years will consume 6.6 g Whey protein + 1.25 g Leucine dissolved in 200 mL of water

Treatment:

Behavioral: Resistance Exercise

Dietary Supplement: 6.6 g Whey protein + 1.25 g leucine

Whey + Leucine + Whey peptides

Experimental group

Description:

After resistance exercise, participants aged 50-79 years will consume 4 g Whey protein + 1.25 g Leucine + 2.6 g Whey peptides dissolved in 200 mL of water

Treatment:

Behavioral: Resistance Exercise

Dietary Supplement: 4 g Whey protein + 1.25 g leucine + 2.6 g Whey peptides

Whey + Leucine + Citrulline

Experimental group

Description:

After resistance exercise, participants aged 50-79 years will consume 6.6 g Whey protein + 1.25 g Leucine + 0.8 g Citrulline dissolved in 200 mL of water

Treatment:

Behavioral: Resistance Exercise

Dietary Supplement: 6.6 g Whey protein + 1.25 g leucine + 0.8 g Citrulline

Water

Sham Comparator group

Description:

After resistance exercise, participants aged either 20-30 or 50-79 years old will consume 200 mL water

Treatment:

Behavioral: Resistance Exercise

Other: Water control