Optimal Protein Dose Per Meal During Weight Loss in Perspective to Maintaining Muscle Mass

University of Aarhus

Status: Completed

Conditions: Protein Metabolism

Treatments: Other: 35 g protein hypocaloric; Other: 35 g protein energy balance; Other: 15 g protein hypocaloric; Other: 60 g protein hypocaloric

Study type: Interventional

Funder types: Other; Industry

Identifiers

NCT03326284

57403

Details and patient eligibility

About

The primary aim of the trial is to determine the optimal dose of protein per meal in relation to enhancing muscle protein synthesis and thereby in perspective counteract muscle loss during weight loss. In addition, we aim to determine whether the response to protein intake is reduced in women on hypocaloric diet compared to in women on an energy-balanced diet.

Full description

Aim of this project is to determine the optimal protein dose (high quality milk protein) per meal in relation to enhancing muscle protein synthesis and thereby counteract muscle loss during weight loss.

The hypothesis is that the optimum dose of protein for women on a hypocaloric diet will be greater than when women eat an energy sufficient diet, and also higher than the optimal dose previously determined in weight stable men.

Methods: Forty women (~ 50 years) will be randomized to four groups. Following a five day hypocaloric diet (three groups) or an energy balanced diet (one group). The women will report to the laboratory to ingest one of three different protein doses. The muscle protein response to the different doses of protein will be determined by the muscle protein fractional synthetic rates using tracer kinetics.

Enrollment: 40 patients

Sex: Female

Ages: 45 to 75 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• healthy
• Postmenopausal women

Exclusion criteria

• Smoking
• Metabolic and/or chronic diseases

Trial design

Primary purpose: Prevention

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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40 participants in 4 patient groups

15g protein hypocaloric diet

Experimental group

Description:

Following a 5-day hypocaloric diet the subjects (n=10) will ingest 15g of protein and their mixed muscle protein synthesis response will be monitored using tracer kinetics.

Treatment:

Other: 15 g protein hypocaloric

35g protein hypocaloric diet

Experimental group

Description:

Following a 5-day hypocaloric diet the subjects (n=10) will ingest 35g of protein and their mixed muscle protein synthesis response will be monitored using tracer kinetics.

Treatment:

Other: 35 g protein hypocaloric

60g protein hypocaloric diet

Experimental group

Description:

Following a 5-day hypocaloric diet the subjects (n=10) will ingest 60g of protein and their mixed muscle protein synthesis response will be monitored using tracer kinetics.

Treatment:

Other: 60 g protein hypocaloric

35g protein energy balanced diet

Experimental group

Description:

Following a 5-day energy balanced diet the subjects (n=10) will ingest 35g of protein and their mixed muscle protein synthesis response will be monitored using tracer kinetics.

Treatment:

Other: 35 g protein energy balance