Optimizing IVF Treatment - the Impact of Time-lapse Culture and Preimplantation Factor (PIF) on Embryo Development.

Regionshospitalet Viborg, Skive

Status: Unknown

Conditions: In Vitro Fertilization; Assisted Reproductive Techniques

Treatments: Procedure: Day 0 denudation and time lapse culture on IVF-oocytes.

Study type: Interventional

Funder types: Other

Identifiers

NCT02222831

Skive-2014-BBP

Details and patient eligibility

About

The aims of this study are to evaluate:

1. the possible effect of time-lapse culture from day zero (day of oocyte retrieval) on fertilisation rates and embryo quality in standard IVF.
2. the association between PIF status in embryo culture medium and embryo quality assessed by standard subjective parameters and morphokinetic development.

Enrollment: 100 estimated patients

Sex: Female

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Standard IVF treatment
• Body mass Index (BMI) between 18 and 30
• couples in which the male partner has normal sperm concentration and motility according to the World Health Organization (WHO, 2010)

Exclusion criteria

• Patients with less than four oocytes

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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100 participants in 2 patient groups

Day 0 - Study arm

Experimental group

Description:

Day 0 denudation and time lapse culture on IVF-oocytes.: After insemination the oocytes will subsequently be denuded and cultured in the EmbryoScope (day 0). The embryo culture media will be collected at day 2-5.

Treatment:

Procedure: Day 0 denudation and time lapse culture on IVF-oocytes.

Day 1 - control arm

No Intervention group

Description:

After insemination oocytes in the control group will be incubated in a standard incubator overnight. On day 1 the oocytes in the will be denuded and further cultured in the EmbryoScope. The embryo culture media will be collected at day 2-5.