Organic Pollutants in Pelvic Endometriosis

Jagiellonian University

Status: Completed

Conditions: Infertility Unexplained; Endometriosis

Treatments: Diagnostic Test: Detection of PAHs in visceral fat with gas chromatography-isotope dilution mass spectrometry

Study type: Interventional

Funder types: Other

Identifiers

NCT05789407

1072.6120.333.2021

Details and patient eligibility

About

The health toxicity of polycyclic aromatic hydrocarbons (PAHs), present in air and food, generated during energy production and waste incineration, is well known. PAHs can activate the aryl hydrocarbon receptor, which may interact with classic estrogen receptors and modify estrogen-dependent inflammation in endometriosis. There is no data on the hypothetical role of PAHs in the etiopathogenesis of endometriosis. The aim was to compare PAHs concentrations in visceral fat in women with endometriosis and idiopathic infertility.

Full description

A prospective cohort tertiary-center study includes women undergoing laparoscopy due to pelvic endometriosis (arm 1) or/and idiopathic infertility (arm 2). A sample of 1 ml of the greater omentum is collected intraoperatively for detection of 16 PAHs, i.e. acenaphthene, acenaphthylene, anthracene, fluoranthene, fluorene, naphthalene, phenanthrene, pyrene, benz[a]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[ghi]perylene, benzo[a]pyrene, chrysene, dibenz[a,h]anthracene, and indeno[1,2,3-cd]pyrene, by gas chromatography-isotope dilution mass spectrometry method. The concentration of selected PAHs in visceral fat in both populations will be compared. The correlation of concentrations of selected PAHs with the occurrence of infertility, pelvic pain syndrome, intensity of pain, peritoneal adhesions and the degree of endometriosis will be checked.

Enrollment: 46 patients

Sex: Female

Ages: 18 to 45 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

i) age 18-45, ii) indications for surgical treatment of endometriosis, iii) indications for invasive diagnostics and surgical treatment of idiopathic infertility.

Exclusion criteria

Trial design

Primary purpose: Diagnostic

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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46 participants in 2 patient groups

endometriosis

Experimental group

Description:

Women subjected to elective laparoscopy for pelvic endometriosis

Treatment:

Diagnostic Test: Detection of PAHs in visceral fat with gas chromatography-isotope dilution mass spectrometry

idiopathic infertility

Active Comparator group

Description:

Women subjected to elective laparoscopy for idiopathic infertility

Treatment:

Diagnostic Test: Detection of PAHs in visceral fat with gas chromatography-isotope dilution mass spectrometry