Osteoclast Impairment in Chronic Periodontitis by Regenerative Materials.

Meenakshi Ammal Dental College and Hospital

Status: Completed

Conditions: Osteoclastic Bone Loss; Periodontal Bone Loss; Periodontal Disease

Treatments: Other: other : PRF

Study type: Interventional

Funder types: Other

Identifiers

NCT03227367

MU-107-IEC/2014

Details and patient eligibility

About

The present study is an effort to investigate the hypothesis that Platelet Rich Fibrin/Biphasic Calcium Phosphate may play a role in inhibiting osteoclasts differentiation and in bone loss.

Full description

Study design

Fifty-five individuals from a suburban population were recruited. Out of them, 5 individuals refused to participate in the study. Finally, 50 participants were selected and divided into two groups, control group [Healthy individuals]: 25 Males/Females, aged 25 to 35 years and test group [Chronic periodontitis patients]: 25 Males/Females aged 30 to 45 years.

Demographic variables and periodontal parameters were measured in both the groups.The investigators explored the effect of Platelet rich fibrin (PRF)/Biphasic calcium phosphate (BCP) on differentiation and survival of osteoclasts obtained from peripheral blood of Chronic Periodontitis patients.

Enrollment: 50 patients

Sex: All

Ages: 25 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

Inclusion criteria: (Control group)

• Subjects with age group between 30 to 45 years.
• Non-smokers.
• No previous history of periodontal treatment.

Inclusion Criteria: (Test group)

• Subjects with age group between 30 to 45 years.
• Presence of atleast 20 teeth
• More than 30% of the sites with clinical attachment loss.
• Non-smokers.
• No previous history of periodontal treatment.

Exclusion Criteria: ( both the groups)

• Individuals with systemic disease
• Subjects under antibiotics or anti-inflammatory drugs within the previous 3 months.
• Pregnant and lactating women.
• Allergic to antibiotics.

Trial design

Primary purpose: Other

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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50 participants in 2 patient groups

Test group

Experimental group

Description:

Mononuclear cells isolated from the peripheral blood of 25 chronic periodontitis patients were interveened with 1ml/well preparation of Platelet rich fibrin(PRF), Biphasic calcium phosphate (BCP) and PRF and BCP combination in-vitro.

Treatment:

Other: other : PRF

control group

Other group

Description:

Mononuclear cells isolated from the peripheral blood of healthy individuals in-vitro.

Treatment:

Other: other : PRF