OX-40 Protein Expression in the Sentinel Lymph Nodes of Patients With Cancer

OHSU Knight Cancer Institute

Status: Completed

Conditions: Cancer

Treatments: Other: laboratory biomarker analysis; Procedure: biopsy; Other: immunohistochemistry staining method; Genetic: protein expression analysis

Study type: Observational

Funder types: Other; NIH

Identifiers

NCT00900302

OHSU-1085

OHSU-SOL-05045-L

P30CA069533 (U.S. NIH Grant/Contract)

CDR0000443230

Details and patient eligibility

About

RATIONALE: Studying protein expression in sentinel lymph node tissue from patients with cancer in the laboratory may help doctors identify and learn more about biomarkers related to cancer. It may also help the study of cancer in the future.

PURPOSE: This laboratory study is evaluating OX-40 protein expression in the sentinel lymph nodes of patients with cancer.

Full description

OBJECTIVES:

• Determine the qualitative and quantitative expression of OX-40 and related markers in sentinel lymph nodes previously removed from patients with cancer.
• Determine the qualitative and quantitative expression of OX-40 and related markers in sentinel lymph nodes freshly removed from patients with cancer.
• Correlate the expression of OX-40 and related markers in sentinel lymph node tissue with expression in nonsentinel tissue from primary tumors and nonsentinel lymph nodes, tumor stage, and patient outcomes.

OUTLINE: Sections of primary tumors, nonsentinel lymph nodes, and sentinel lymph nodes will be analyzed for expression of OX-40 by immunohistochemistry. Sections of negative (no tumor metastases) lymph nodes will be used as internal controls. Fresh tissue and blood will be tested for marker analysis and in vitro assays of immune function.

PROJECTED ACCRUAL: A total of 100 specimens from sentinel lymph nodes previously removed from cancer patients and 100 specimens from sentinel lymph nodes freshly removed from cancer patients will be accrued for this study.

Enrollment: 98 patients

Sex: All

Ages: Under 120 years old

Volunteers: No Healthy Volunteers

Inclusion and exclusion criteria

DISEASE CHARACTERISTICS:

• Histologically or cytologically confirmed cancer, including, but not limited to, the following:

  • Melanoma

  • Breast cancer

  • Invasive squamous cell cancer

  • Other cancers for which patients underwent or will undergo the following:

    • Adequate archived paraffin-embedded tissue for making slides for immunostaining
    • Adequate freshly removed sentinel lymph node (and primary tumor, if available) tissue for laboratory assays

• Clinical data and date of last follow-up on tumor stage and status must be available

• Hormone receptor status not specified

PATIENT CHARACTERISTICS:

• Not specified

PRIOR CONCURRENT THERAPY:

• Not specified