Oxidative and Antioxidant Indices to HIIT and Aloe Vera Supplementation in Patients With T2DM

University of Nis

Status

Completed

Conditions

Diabetes Type 2

Treatments

Behavioral: Exercise

Dietary Supplement: Aloe vera

Study type

Interventional

Funder types

Other

Identifiers

NCT06377657

DONT4GET

Details and patient eligibility

About

Diabetes is a prevalent metabolic disease that leads to increased blood sugar levels due to insulin shortage or resistance. Type 2 diabetes is often linked to obesity, which can increase insulin resistance. Meteorin-like protein (METRNL) is a new secreted protein that affects insulin sensitivity and has been found to be negatively related to serum glucose levels in people with diabetes.

Oxidative stress in diabetics can stimulate the production of inflammatory mediators, increasing the activity of the antioxidant system. Aloe vera, a widely used plant, has been used for treating diabetes, wound healing, tumors, and inflammatory bowel disease. It has anti-inflammatory, antioxidant, neuroprotective, antidepressant, and memory-enhancing effects.

Exercise can improve diabetic patients' conditions by increasing the expression of GLUT4 protein and skeletal muscle. High-intensity interval training has been shown to increase the expression of the METORIN gene in human subjects and decrease MDA in type 2 diabetic patients. However, no studies have examined the simultaneous effect of exercise and Aloe vera on diabetes indicators.

The present study aims to investigate the response of METRNL and some oxidative and antioxidant plasma indicators to high-intensity interval training and Aloe vera supplementation in type 2 diabetic men. The findings indicate that exercise and consumption of plants with anti-inflammatory and antioxidant properties can be effective in modulating the pathological effects of diabetes and increasing insulin sensitivity.

Enrollment

44 patients

Sex

Male

Ages

25 to 40 years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Male participants who have been diagnosed with T2DM at least 3 months prior to the start of the study according to standard criteria
Inactive participants according to the International Physical Activity Questionnaire (IPAQ)
Participants receiving stable medical therapy for T2DM, excluding those on exogenous insulin therapy
Participants who were free from injuries or chronic health conditions that could limit their ability to safely participate in an intense exercise program were included in the study

Exclusion criteria

Fasting glucose above 270 mg / dL
Exogenous insulin
History of diabetic complications (the final stage of proliferative diabetic retinopathy, or subsequent overt nephropathy, diabetic ketoacidosis, or severe diabetic neuropathy)
Hereditary glucose and galactose malabsorption

Trial design

Primary purpose

Treatment

Allocation

Randomized

Interventional model

Parallel Assignment

Masking

Double Blind

44 participants in 4 patient groups

High-Intensity Interval Training

Experimental group

Description:

Participants in HIIT group underwent 3 sessions per week for 12 weeks. The HIIT protocol (maximum 60 cycles per 20-minute session) includes 8-second fast pedaling (approximately 80% of maximum heart rate (HR max)) followed by slow pedaling (20-30 rpm) for 12 seconds. To facilitate acceleration and limit the stagnation of the bicycle wheel, the selected resistance was very low (almost zero).

Treatment:

Behavioral: Exercise

Aloe Vera supplementation

Experimental group

Description:

Participants in Aloe Vera group underwent supplementation with Aloe vera capsule containing 100 mg of Aloe vera gel powder in the morning immediately after waking up and one capsule after dinner.

Treatment:

Dietary Supplement: Aloe vera

HIIT + Aloe Vera

Experimental group

Description:

Treatment:

Behavioral: Exercise

Dietary Supplement: Aloe vera

Control group

No Intervention group

Description:

The control group continued their daily life activities for 12 weeks